Origin of mandibular condylar cartilage in mice, rats, and humans: Periosteum or separate blastema?
Origin of mandibular condylar cartilage in mice, rats, and humans: Periosteum or separate blastema?
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DOI：
10.1016/j.job.2013.08.001
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发表时间：
2013-11-01
影响因子：
2.4
通讯作者：
Francisco Rodriguez-Vazquez, Jose
中科院分区：
文献类型：
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作者：
Shibata, Shunichi;Sato, Rei;Francisco Rodriguez-Vazquez, Jose
目的：探讨下颌髁突软骨是来自骨化的下颌骨骨膜还是来自一个独立的、程序化的母胚。材料与方法：采用妊娠14.0 ~ 16.0胎鼠、妊娠16.0 ~ 18.0胎鼠和妊娠9、10周人胚胎。采用连续切片法和酶组化法检测大鼠髁突软骨初始形成过程中碱性磷酸酶活性。我们还分析了人类胎儿连续切片的组织学观察和三维重建模型。通过原位杂交直接比较大鼠和小鼠髁突软骨中胶原型mRNA的表达。结果：在E16.0时，在骨化的下颌骨后位清晰地识别出大鼠髁突（髁突）的一个突起，并与之连续。[16.5]观察到新形成的大鼠髁软骨，与骨化的下颌骨连续。[16.0]时髁突软骨间充质细胞显示碱性磷酸酶活性，新形成的髁突软骨细胞也显示酶活性。因此，从碱性磷酸酶阳性骨膜样细胞衍生的大鼠下颌髁软骨与骨化的下颌骨是连续的，正如先前在小鼠中所证明的那样，但与小鼠相比，大鼠向增生性软骨细胞的快速分化并不显著。在人胚胎中，髁突和新形成的软骨与骨化的下颌骨是连续的。结论：哺乳动物下颌骨髁突软骨来源于小鼠、大鼠和人类骨化下颌骨的骨膜。(C) 2013年日本口腔生物学会。Elsevier B.V.版权所有。
Objective: To investigate if mandibular condylar cartilage is derived from the periosteum of the ossifying mandible or from a separate, programmed blastema.Materials and methods: Fetal mice at E14.0-16.0, fetal rats at E16.0-18.0, and human embryos at 9 and 10 wks of gestation were used. The initial formation of rat condylar cartilage was investigated by using serial sections and enzyme-histochemistry to detect alkaline phosphatase activity. Histological observations of serial sections of human fetuses as well as 3D -reconstruction models were also analyzed. The expression of collagen type mRNA in developing rat condylar cartilage was directly compared with that in mice by performing in situ hybridization.Results: An anlage of the rat condylar process (condylar anlage) was clearly identified in the posterior position of the ossifying mandible and was continuous with it at E16.0. Newly formed rat condylar cartilage was observed at [16.5 and was continuous with the ossifying mandible. Mesenchymal cells in the condylar anlage at [16.0 showed alkaline phosphatase activity and chondrocytes in the newly formed condylar cartilage also showed enzymatic activity. Thus, rat mandibular condylar cartilage that derives from alkaline phosphatase-positive periosteum-like cells is continuous with the ossifying mandible, as previously demonstrated in mice, but rapid differentiation into hypertrophic chondrocytes in rats is not remarkable compared to that in mice. The condylar anlage and the newly formed cartilage were also continuous with the ossifying mandible in human embryos.Conclusions: Mammalian mandibular condylar cartilage derives from the periosteum of the ossifying mandible in mice, rats, and humans. (C) 2013 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.