Identification of the early leaf senescence gene ELS3 in bread wheat (Triticum aestivum L.)
Identification of the early leaf senescence gene ELS3 in bread wheat (Triticum aestivum L.)
复制标题
DOI：
10.1007/s00425-023-04278-x
复制
发表时间：
2024-01-01
期刊：
影响因子：
4.3
通讯作者：
Zhang，Lichao
中科院分区：
文献类型：
--
作者：
Xie，Zhencheng;Zhang，Qiang;Zhang，Lichao
主要结论小麦叶片衰老突变体3的特征及其致病基因ELS 3的鉴定摘要叶片衰老是影响作物产量和品质的重要农艺性状。然而，很少有小麦衰老相关基因的克隆和功能分析。在这里，我们报告了小麦早期叶片衰老突变体的特征和其致病基因ELS 3的精细定位。与野生型烟占4110（YZ 4110）相比，突变体els 3开花后叶绿素含量下降，叶绿体结构退化。进一步的生理生化分析表明，在ELS 3突变体中，开花后旗叶超氧阴离子和过氧化氢含量逐渐增加，而过氧化氢酶、超氧化物歧化酶和谷胱甘肽还原酶等抗氧化酶活性逐渐降低。为了克隆叶片衰老表型的致病基因，利用F2：3群体的10,133个个体构建了一张遗传图谱，将ELS 3定位于2DL染色体上2.52 Mb的区域，包含16个推测的基因。随后的序列分析和基因注释发现，TraesCS 2D 02 G332700的第一外显子仅有一个SNP（C → T），导致一个氨基酸替换（Pro329 Ser），初步认为TraesCS 2D 02 G332700是ELS 3的候选基因。ELS 3编码一种定位于细胞膜上的富含亮氨酸重复序列的受体样激酶（LRR-RLK）蛋白。我们还发现突变体TraesCS 2D 02 G332700的瞬时表达可以诱导烟草叶片衰老。本萨米亚纳综合分析，TraesCS 2D 02 G332700可能是ELS 3的候选基因，可能具有调控叶片衰老的功能。
Main conclusionCharacterization of the early leaf senescence mutantels3and identification of its causal geneELS3, which encodes an LRR-RLK protein in wheat.AbstractLeaf senescence is an important agronomic trait that affects both crop yield and quality. However, few senescence-related genes in wheat have been cloned and functionally analyzed. Here, we report the characterization of the early leaf senescence mutantels3and fine mapping of its causal geneELS3in wheat. Compared with wild-type Yanzhan4110 (YZ4110), theels3mutant had a decreased chlorophyll content and a degraded chloroplast structure after the flowering stage. Further biochemical assays in flag leaves showed that the superoxide anion and hydrogen peroxide contents increased, while the activities of antioxidant enzymes, including catalase, superoxide dismutase and glutathione reductase, decreased gradually after the flowering stage in theels3mutant. To clone the causal gene underlying the phenotype of leaf senescence, a genetic map was constructed using 10,133 individuals of F2:3populations, andELS3was located in a 2.52 Mb region on chromosome 2DL containing 16 putative genes. Subsequent sequence analysis and gene annotation identified only one SNP (C to T) in the first exon ofTraesCS2D02G332700,resulting in an amino acid substitution (Pro329Ser), andTraesCS2D02G332700was preliminarily considered as the candidate gene ofELS3.ELS3encodes a leucine-rich repeat receptor-like kinase (LRR-RLK) protein that is localized on the cell membrane. We also found that the transient expression of mutantTraesCS2D02G332700can induce leaf senescence inN. benthamiana. Taken together,TraesCS2D02G332700is likely to be the candidate gene ofELS3and may have a function in regulating leaf senescence.