Human amnion contains a novel laminin variant, laminin 7, which like laminin 6, covalently associates with laminin 5 to promote stable epithelial-stromal attachment.
Human amnion contains a novel laminin variant, laminin 7, which like laminin 6, covalently associates with laminin 5 to promote stable epithelial-stromal attachment.
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人羊膜含有一种新型的层粘连蛋白变体7，与层粘连蛋白6一样，将其与层粘连蛋白5共同促进，以促进稳定的上皮粘膜附着。
DOI：
10.1083/jcb.132.6.1189
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发表时间：
1996-03
影响因子：
7.8
通讯作者：
Burgeson, RE
中科院分区：
文献类型：
--
作者：
Champliaud, MF;Lunstrum, GP;Rousselle, P;Nishiyama, T;Keene, DR;Burgeson, RE
外上皮细胞与基底膜和下层基质的稳定附着是由跨膜蛋白介导的，如沿上皮细胞基底外侧表面的半粒内的整合素alpha6beta4和大疱性天疱疮抗原2，以及它们的配体，包括一个特殊的层粘连蛋白亚家族。层粘连蛋白5分子（以前称为kalinin/nicein/epiligrin）是这个上皮特异性亚家族的成员。层粘连蛋白5链不仅在III-VI结构域内被大量截断，而且在体外和体内也被广泛地进行蛋白水解处理。因此，在成熟的层粘连蛋白5分子中缺乏层粘连蛋白与其他基底膜组分结合所需的结构域。因此，层粘连蛋白5与基膜的紧密结合可能是通过一种独特的机制发生的。为了检测组织中的层粘连蛋白5，我们选择人羊膜作为来源，因为它的可用性和羊膜上皮基底膜与皮肤的相似性。我们从人羊膜基底膜中分离出层粘连蛋白5。除了单体层粘连蛋白5外，我们发现分离的大部分层粘连蛋白5与层粘连蛋白6 （alpha3beta1gamma1）和一种新的层粘连蛋白同型（我们称之为层粘连蛋白7 (alpha3beta2gamma1)）共价内合。我们认为层粘连蛋白5与层粘连蛋白6和7的结合是羊膜中层粘连蛋白5与基膜稳定结合的一种机制。通过免疫荧光可以在人羊膜上皮-间质界面以及胎牛和成年牛皮肤的真皮-表皮交界处看到β 2链，但在新生儿皮肤中不存在，或仅微弱存在。
Stable attachment of external epithelia to the basement membrane and underlying stroma is mediated by transmembrane proteins such as the integrin alpha6beta4 and bullous pemphigoid antigen 2 within the hemidesmosomes along the basolateral surface of the epithelial cell and their ligands that include a specialized subfamily of laminins. The laminin 5 molecule (previously termed kalinin/nicein/epiligrin) is a member of this epithelial-specific subfamily. Laminin 5 chains are not only considerably truncated within domains III-VI, but are also extensively proteolytically processed in vitro and in vivo. As a result, the domains expected to be required for the association of laminins with other basement membrane components are lacking in the mature laminin 5 molecule. Therefore, the tight binding of laminin 5 to the basement membrane may occur by a unique mechanism. To examine laminin 5 in tissue, we chose human amnion as the source, because of its availability and the similarity of the amniotic epithelial basement membrane with that of skin. We isolated the laminin 5 contained within the basement membrane of human amnion. In addition to monomeric laminin 5, we find that much of the laminin 5 isolated is covalently adducted with laminin 6 (alpha3beta1gamma1) and a novel laminin isotype we have termed laminin 7 (alpha3beta2gamma1). We propose that the association between laminin 5 and laminins 6 and 7 is a mechanism used in amnion to allow stable association of laminin 5 with the basement membrane. The beta2 chain is seen at the human amniotic epithelial-stromal interface and at the dermal-epidermal junction of fetal and adult bovine skin by immunofluorescence, but is not present, or only weakly present, in neonatal human skin.
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整联蛋白α3β1在局灶性粘连中的不同功能和α6β4/bullous子型抗原抗原在新的稳定锚定接触（SAC）的角质形成细胞中：与Hemidesmosmosys的关系。
影响因子：
7.8
作者：
Carter, W G;Kaur, P;Gil, S G;Gahr, P J;Wayner, E A
通讯作者：
Wayner, E A
DOI：
10.1073/pnas.84.20.6970
发表时间：
1987-10-01
影响因子：
11.1
作者：
AEBERSOLD, RH;LEAVITT, J;KENT, SBH
通讯作者：
KENT, SBH
DOI：
10.1091/mbc.1.10.731
发表时间：
1990-09-01
期刊：
CELL REGULATION
影响因子：
--
作者：
ENGVALL, E;EARWICKER, D;SANES, JR
通讯作者：
SANES, JR
影响因子：
7.8
作者：
KEENE, DR;SAKAI, LY;BURGESON, RE
通讯作者：
BURGESON, RE
DOI：
10.1083/jcb.119.3.679
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kallunki P;Sainio K;Eddy R;Byers M;Kallunki T;Sariola H;Beck K;Hirvonen H;Shows TB;Tryggvason K
通讯作者：
Tryggvason K