Development of tolerance to ethanol in relation to the alcohol dehydrogenase locus in Drosophila melanogaster 1. Adult and egg-to-adult survival in relation to ADH activity
Development of tolerance to ethanol in relation to the alcohol dehydrogenase locus in Drosophila melanogaster 1. Adult and egg-to-adult survival in relation to ADH activity
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黑腹果蝇乙醇脱氢酶位点的乙醇耐受性发展 1. 成虫和卵至成虫存活率与 ADH 活性的关系
DOI：
10.1038/hdy.1985.118
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发表时间：
1985
期刊：
影响因子：
3.8
通讯作者：
W. van Delden
中科院分区：
文献类型：
--
作者：
J. Kerver;W. van Delden
将AdhF或AdhS等位基因纯合的黑腹果蝇品系保持在补充有乙醇的食物上。90代后，这些菌株（FFE和SSE）进行了测试，以乙醇的耐受性，并与对照菌株（FFN和SSN）从普通食品进行了比较。E菌株表现出增加的耐受性乙醇，无论是在成年人和青少年的生命阶段。在成人中，耐受性的增加并不伴随着ADH活性的增加。在青少年的生活阶段有显着差异，ADH活性之间的E和N株，无论是在普通食品和含乙醇的食品。然而，ADH活性是不是唯一的因素参与增加的耐受性乙醇。母亲的Adh基因型是至关重要的，因为母亲的影响，乙醇耐受性的鸡蛋和一龄幼虫的鸡蛋到成人的生存。此外，E菌株的发育时间比相应的N菌株长。它的结论是，适应含乙醇的食物中没有实现以同样的方式在FFE和SSE菌株。
Strains of Drosophila melanogaster homozygous either for the AdhF or the AdhS allele, were kept on food supplemented with ethanol. After 90 generations these strains (FFE and SSE) were tested for tolerance to ethanol and compared with control strains (FFN and SSN) from regular food. The E strains showed increased tolerance to ethanol, both in the adult and in the juvenile life stages. In the adults the increase in tolerance was not accompanied by an increase in ADH activity. In the juvenile life stages there were significant differences in ADH activity between the E and N strains, both on regular food and on food containing ethanol. However, ADH activity was not the only factor involved in the increased tolerance to ethanol.The Adh genotype of the mother was of paramount importance to egg-to-adult survival because of a maternal effect on ethanol tolerance in the eggs and first instar larvae. Furthermore developmental times of the E strains were longer than those of the corresponding N strains. It is concluded that the adaptation to ethanol-containing food was not realised in the same way in the FFE and SSE strains.