Evolution and single-nucleotide polymorphisms in methicillin-resistant Staphylococcus aureus strains with reduced susceptibility to vancomycin and daptomycin, based on determination of the complete genome.
Evolution and single-nucleotide polymorphisms in methicillin-resistant Staphylococcus aureus strains with reduced susceptibility to vancomycin and daptomycin, based on determination of the complete genome.
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基于完整基因组的测定，对万古霉素和达托霉素敏感性降低的耐甲氧西林金黄色葡萄球菌菌株的进化和单核苷酸多态性。
DOI：
10.1128/aac.05159-14
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Matsumoto T.
中科院分区：
文献类型：
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作者：
Yamaguchi T;Suzuki S;Okamura S;Miura Y;Tsukimori A;Nakamura I;Ito N;Masuya A;Shiina T;Matsumoto T.
我们从一位患者身上获得了一系列耐甲氧西林金黄色葡萄球菌分离株，包括达托霉素敏感菌株TD1和耐达托霉素菌株TD4。我们利用新一代测序技术确定了TD1和TD4的全基因组序列，仅鉴定出4个单核苷酸多态性（snp），分别为capb （E58K）、rpoB（H481Y）、lytN（I16V）和mprf （V351E）。我们确定这四个snp足以导致菌株产生达托霉素、万古霉素和利福平耐药。
We obtained a series of methicillin-resistant Staphylococcus aureus isolates, including both daptomycin-susceptible strain TD1 and daptomycin-resistant strain TD4, from a patient. We determined the complete genome sequences of TD1 and TD4 using next-generation sequencing, and only four single-nucleotide polymorphisms (SNPs) were identified, one each incapB(E58K),rpoB(H481Y),lytN(I16V), andmprF(V351E). We determined that these four SNPs were sufficient to cause the strains to develop daptomycin, vancomycin, and rifampin resistance.