Stepwise evolution of the centriole-assembly pathway
Stepwise evolution of the centriole-assembly pathway
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DOI：
10.1242/jcs.064931
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发表时间：
2010-05-01
影响因子：
4
通讯作者：
Bettencourt-Dias, Monica
中科院分区：
文献类型：
--
作者：
Carvalho-Santos, Zita;Machado, Pedro;Bettencourt-Dias, Monica
中心粒和基体（CBB）结构使纤毛和鞭毛成核，并且是中心体的重要组成部分，是真核生物基于微管的运动、细胞分裂和极性的基础。近年来，CBB组装机制的组成部分已被确定，但对它们的调控和进化知之甚少。考虑到真核生物中遇到的细胞背景的多样性，但CBB形态的显着保守性，我们问是否一般的机械原理可以解释CBB组装。我们分析了人类CBB组装机器的每个组件在真核生物中的分布，作为生成可验证假设的策略。我们发现了一个进化上有凝聚力的祖先模块，我们称之为UNIMOD，由三个组件（SAS 6，SAS 4/CPAP和BLD 10/CEP 135）定义，与CBB的发生相关。出乎意料的是，其他参与者（SAK/PLK 4，SPD 2/CEP 192和CP 110）以分类特异性的方式出现。我们报告说，基因重复在CBB组件的进化中起着重要的作用，并表明，在BLD 10/CEP 135的情况下，这是CBB和鞭毛生物发生的组织特异性的来源。此外，我们观察到CBB组件之间的极端蛋白质的分歧，并通过实验表明，SAK/PLK 4家族成员之间存在跨物种互补的损失，这表明CBB组装中的物种特异性适应。我们建议，UNIMOD理论解释CBB架构的保护和分类和组织特异性的分子创新，通过出现，复制和分歧，协调CBB生物发生和功能在不同的细胞环境中发挥重要作用。
The centriole and basal body (CBB) structure nucleates cilia and flagella, and is an essential component of the centrosome, underlying eukaryotic microtubule-based motility, cell division and polarity. In recent years, components of the CBB-assembly machinery have been identified, but little is known about their regulation and evolution. Given the diversity of cellular contexts encountered in eukaryotes, but the remarkable conservation of CBB morphology, we asked whether general mechanistic principles could explain CBB assembly. We analysed the distribution of each component of the human CBB-assembly machinery across eukaryotes as a strategy to generate testable hypotheses. We found an evolutionarily cohesive and ancestral module, which we term UNIMOD and is defined by three components (SAS6, SAS4/CPAP and BLD10/CEP135), that correlates with the occurrence of CBBs. Unexpectedly, other players (SAK/PLK4, SPD2/CEP192 and CP110) emerged in a taxon-specific manner. We report that gene duplication plays an important role in the evolution of CBB components and show that, in the case of BLD10/CEP135, this is a source of tissue specificity in CBB and flagella biogenesis. Moreover, we observe extreme protein divergence amongst CBB components and show experimentally that there is loss of cross-species complementation among SAK/PLK4 family members, suggesting species-specific adaptations in CBB assembly. We propose that the UNIMOD theory explains the conservation of CBB architecture and that taxon-and tissue-specific molecular innovations, gained through emergence, duplication and divergence, play important roles in coordinating CBB biogenesis and function in different cellular contexts.