Characterization of the polyphenol oxidase gene family reveals a novel microRNA involved in posttranscriptional regulation of PPOs in Salvia miltiorrhiza.
Characterization of the polyphenol oxidase gene family reveals a novel microRNA involved in posttranscriptional regulation of PPOs in Salvia miltiorrhiza.
复制标题
多酚氧化酶基因家族的表征揭示了一种参与丹参 PPO 转录后调控的新型 microRNA
DOI：
10.1038/srep44622
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发表时间：
2017-03-17
影响因子：
4.6
通讯作者：
Lu S
中科院分区：
文献类型：
--
作者：
Li C;Li D;Li J;Shao F;Lu S
丹参是一种著名的中药原料。了解酚酸生物合成和代谢的调控机制对S.丹参质量改进我们在这里报告说，S。丹参含有19个多酚氧化酶（PPO）基因，是已知植物中最大的PPO基因家族。基因结构和序列特征分析揭示了SmPPOS的保守性和差异性。SmPPOS在植物组织中差异表达，其中8个主要表达于韧皮部和木质部，表明一些SmPPOS在功能上是冗余的，而另一些SmPPOS则与不同的生理过程有关。18个SmPPOs的表达模式在MeJA处理下显著改变，12个是酵母提取物和Ag+响应的，这表明大多数SmPPOs是应激响应的。高通量小RNA序列和降解组数据的分析表明，存在于大果杨中的PPOs的miR 1444介导的调节不存在于S.丹参。相反，SmPPOs的一个子集是由一种新的miRNA，称为Smi-miR 12112转录后调节的。这表明了miRNA介导的PPO调控的特异性和意义。这些结果揭示了SmPPO表达的调控，并表明SmPPO相关的酚酸生物合成和代谢的复杂性。
Salvia miltiorrhiza is a well-known material of traditional Chinese medicine. Understanding the regulatory mechanisms of phenolic acid biosynthesis and metabolism are important for S. miltiorrhiza quality improvement. We report here that S. miltiorrhiza contains 19 polyphenol oxidases (PPOs), forming the largest PPO gene family in plant species to our knowledge. Analysis of gene structures and sequence features revealed the conservation and divergence of SmPPOs. SmPPOs were differentially expressed in plant tissues and eight of them were predominantly expressed in phloem and xylem, indicating that some SmPPOs are functionally redundant, whereas the others are associated with different physiological processes. Expression patterns of eighteen SmPPOs were significantly altered under MeJA treatment, and twelve were yeast extract and Ag+-responsive, suggesting the majority of SmPPOs are stress-responsive. Analysis of high-throughput small RNA sequences and degradome data showed that miR1444-mediated regulation of PPOs existing in P. trichocarpa is absent from S. miltiorrhiza. Instead, a subset of SmPPOs was posttranscriptionally regulated by a novel miRNA, termed Smi-miR12112. It indicates the specificity and significance of miRNA-mediated regulation of PPOs. The results shed light on the regulation of SmPPO expression and suggest the complexity of SmPPO-associated phenolic acid biosynthesis and metabolism.