Identification of members of gene families in Arabidopsis thaliana by contig construction from partial cDNA sequences: 106 genes encoding 50 cytoplasmic ribosomal proteins
Identification of members of gene families in Arabidopsis thaliana by contig construction from partial cDNA sequences: 106 genes encoding 50 cytoplasmic ribosomal proteins
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DOI：
10.1046/j.1365-313x.1997.11051127.x
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发表时间：
1997-05-01
期刊：
影响因子：
7.2
通讯作者：
Delseny, M
中科院分区：
文献类型：
--
作者：
Cooke, R;Raynal, M;Delseny, M
部分cDNA测序获得的表达序列标签（EST）已导致标签的约8000的估计20000基因在拟南芥中的鉴定。这一数字是国际数据库中该生物体完整编码序列数量的四到五倍。与哺乳动物不同，A. thaliana.以核糖体蛋白质基因家族为例，可以从重叠EST构建相对长的序列，这些序列具有足够高的质量，能够明确地识别多基因家族单个成员的标签，即使序列高度保守。已经鉴定了总共106个编码50种不同细胞质核糖体蛋白类型的基因，大多数蛋白质由至少两个和多达四个基因编码。单个基因家族成员的编码序列几乎总是非常高度保守的，并且在绝大多数情况下，衍生的氨基酸序列即使不完全相同，也几乎相同。在非翻译区观察到序列差异，这允许定义基因特异性探针。该方法可用于构建任何蛋白质的高质量标签。
Partial cDNA sequencing to obtain expressed sequence tags (ESTs) has led to the identification of tags to about 8000 of the estimated 20 000 genes in Arabidopsis thaliana. This figure represents four to five times the number of complete coding sequences from this organism available in international databases. In contrast to mammals, many proteins are encoded by multigene families in A. thaliana. Using ribosomal protein gene families as an example, it is possible to construct relatively long sequences from overlapping ESTs which are of sufficiently high quality to be able to unambiguously identify tags to individual members of multigene families, even when the sequences are highly conserved. A total of 106 genes encoding 50 different cytoplasmic ribosomal protein types have been identified, most proteins being encoded by at least two and up to four genes. Coding sequences of members of individual gene families are almost always very highly conserved and derived amino acid sequences are almost, if not completely, identical in the vast majority of cases. Sequence divergence is observed in untranslated regions which allows the definition of gene-specific probes. The method can be used to construct high-quality tags to any protein.