Coaxial PCL/PEG-thiol-ene microfiber with tunable physico-chemical properties for regenerative scaffolds
Coaxial PCL/PEG-thiol-ene microfiber with tunable physico-chemical properties for regenerative scaffolds
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DOI：
10.1039/c9bm00388f
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发表时间：
2019-09-01
影响因子：
6.6
通讯作者：
Tan, Wei
中科院分区：
文献类型：
--
作者：
Iglesias-Echevarria, Monica;Durante, Luca;Tan, Wei
组织再生需要表现出与待替换的组织相似的机械性质的支架，同时允许细胞浸润和细胞外基质产生。理想情况下，支架的多孔结构和物理化学性质可以精确定义，以满足再生需求。因此，我们开发的技术，以生产混合纤维同轴结构的聚己内酯芯和4臂，聚乙二醇硫醇-异丙基苯鞘。我们评估了交联密度和鞘聚合物尺寸对支架结构、物理和机械性能以及体外和体内细胞-支架相互作用的各自影响。所有支架均显示出高弹性、溶胀和强度，模仿软组织特性。重要的是，硫醇-烯水凝胶鞘能够实现可调的柔软性和肽系留以用于细胞活性。随着光聚合的增加，由于纤维内和纤维间的交联，发现支架的硬化和溶胀减少。更多聚合的支架还增强了体外细胞-支架相互作用，并诱导自发的深层细胞浸润，以产生用于体内组织再生的胶原蛋白和弹性蛋白。鞘聚合物的分子量提供了改变支架的物理性质和生物活性的额外机制。总的来说，这些具有可调弹性和再生线索的坚固支架为组织再生提供了一个多功能和有效的平台。
Tissue regeneration requires scaffolds that exhibit mechanical properties similar to the tissues to be replaced while allowing cell infiltration and extracellular matrix production. Ideally, the scaffolds' porous architecture and physico-chemical properties can be precisely defined to address regenerative needs. We thus developed techniques to produce hybrid fibers coaxially structured with a polycaprolactone core and a 4-arm, polyethylene glycol thiol-norbornene sheath. We assessed the respective effects of crosslink density and sheath polymer size on the scaffold architecture, physical and mechanical properties, as well as cell-scaffold interactions in vitro and in vivo. All scaffolds displayed high elasticity, swelling and strength, mimicking soft tissue properties. Importantly, the thiol-ene hydrogel sheath enabled tunable softness and peptide tethering for cellular activities. With increased photopolymerization, stiffening and reduced swelling of scaffolds were found due to intra- and inter-fiber crosslinking. More polymerized scaffolds also enhanced the cell-scaffold interaction in vitro and induced spontaneous, deep cell infiltration to produce collagen and elastin for tissue regeneration in vivo. The molecular weight of sheath polymer provides an additional mechanism to alter the physical properties and biological activities of scaffolds. Overall, these robust scaffolds with tunable elasticity and regenerative cues offered a versatile and effective platform for tissue regeneration.