Diet factors responsible for the change of the glucose oxidase activity in labial salivary glands of Helicoverpa armigera
Diet factors responsible for the change of the glucose oxidase activity in labial salivary glands of Helicoverpa armigera
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饮食因素影响棉铃虫唇唾液腺葡萄糖氧化酶活性的变化
DOI：
10.1002/arch.20240
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发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
--
作者：
Hu, Yong-Hong;Leung, David W. M.;Wang, Chen-Zhu
本文研究了取食人工饲料和寄主植物烟草后棉铃虫（Helicoverpaarmigera）幼虫唇腺葡萄糖氧化酶（GOX）活性的变化及其影响因素。在整个幼虫发育过程中，人工饲料饲养的幼虫唇唾液GOX活性显著高于植物饲养的幼虫。五龄幼虫取食人工饲料后，其唇唾液GOX活性迅速升高，且与取食时间密切相关。人工饲料的总糖含量是烟叶的68倍。我们假设糖和次级代谢产物是诱导GOX活性的可能原因。当取食涂有葡萄糖或蔗糖的烟叶时，五龄幼虫唇唾液GOX活性显著高于取食未涂糖烟叶的幼虫。非变性PAGE后，在所有喂食不同饲料的毛毛虫中均观察到唇唾液GOX的I个单键，这意味着只有同工酶的活性因饲料不同而发生变化。此外，用含有绿原酸、芦丁和槲皮素的人工饲料喂养毛虫后，测定了毛虫唇唾液GOX活性。结果表明，这些酚类化合物对GOX活性没有影响。我们的结论是，在饮食中的糖是影响唇唾液GOX活性的幼虫的一个主要因素。
We investigated the change of the glucose oxidase (GOX) activity in labial salivary glands of Helicoverpa armigera larvae fed with the artificial diet or host plant tobacco and the major factors responsible for such a change. Throughout larval development, the labial salivary GOX activities in caterpillars reared on the artificial diet were remarkably higher than those fed with the plant. After fifth-instar plant-fed caterpillars were transferred to the artificial diet, their labial salivary GOX activity increased quickly, which was closely correlated with the time spent feeding on the artificial diet. The total sugar content of the artificial diet was 68 times higher than that of the tobacco leaves. We hypothesized that sugars and secondary metabolites are the possible causes of induction of GOX activity. When fifth-instar caterpillars were fed with tobacco leaves coated with glucose or sucrose, their labial salivary GOX activity was significantly higher than those fed with leaves without sugar coating. Following native PAGE, I single bond of the labial salivary GOX was observed in all the caterpillars fed with different diets, implying that only the activity of the isoenzyme was changed in response to different diets. Furthermore, the labial salivary GOX activity was determined after caterpillars were fed with artificial diets containing chlorogenic acid, rutin, and quercetin. The results showed that all these phenolic compounds had no effect on the GOX activity. We conclude that sugar in diets was a major factor influencing the labial salivary GOX activity of the larvae.