Determination of delta-aminolaevulinic acid in biological fluids by gas-liquid chromatography with electron-capture detection.
Determination of delta-aminolaevulinic acid in biological fluids by gas-liquid chromatography with electron-capture detection.
复制标题
采用电子捕获检测气相色谱法测定生物体液中的 δ-氨基乙酰丙酸。
DOI：
10.1042/bj2190883
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
A. Gorchein
中科院分区：
文献类型：
--
作者：
A. Gorchein
δ-氨基乙酰丙酸（AmLev）的衍生物，2-甲基-3-乙酰基-4-（3-丙酸五氟苄基酯）吡咯，具有良好的g.l.c.电子捕获检测，合成。在柱上可以检测到小于1 pg。6-氨基-5-氧代己酸在类似条件下形成类似衍生物，并用作开发AmLev高灵敏度和特异性测定的内标物。该方法已被应用于外周静脉和脐带血浆和脑脊液的正常和卟啉受试者。
A derivative of delta-aminolaevulinic acid (AmLev), 2-methyl-3-acetyl-4-(3-propionic acid pentafluorobenzyl ester)pyrrole, with favourable properties for g.l.c. with electron-capture detection, was synthesized. Less than 1 pg could be detected on the column. 6-Amino-5-oxohexanoic acid formed the analogous derivative under similar conditions and was used as the internal standard in the development of a highly sensitive and specific assay for AmLev. The method has been applied to peripheral-venous and umbilical-cord plasma and to cerebrospinal fluid of normal and porphyric subjects.