PROTEIN-SYNTHESIS REQUIRES CELL-SURFACE CONTACT WHILE NUCLEAR EVENTS RESPOND TO CELL-SHAPE IN ANCHORAGE-DEPENDENT FIBROBLASTS
PROTEIN-SYNTHESIS REQUIRES CELL-SURFACE CONTACT WHILE NUCLEAR EVENTS RESPOND TO CELL-SHAPE IN ANCHORAGE-DEPENDENT FIBROBLASTS
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DOI：
10.1016/0092-8674(80)90473-0
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
PENMAN, S
中科院分区：
文献类型：
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作者：
BENZEEV, A;FARMER, SR;PENMAN, S
锚定依赖性小鼠3 T6成纤维细胞只有在附着并扩散在固体基质上时才能生长。甲基纤维素中的细胞悬浮液导致主要RNA和蛋白质合成系统的显著协调抑制，并且当细胞重新接种在组织培养皿表面上时，这些系统依次恢复。通过控制细胞形状，将细胞再附着对代谢的影响与随后的扩散的影响分开。大分子代谢首先受到长期悬浮培养的强烈抑制。然后在存在多种扩散抑制剂的情况下将细胞重新铺板。重新附着后蛋白质合成的恢复很快，不需要广泛的细胞扩散。质膜的有限部分与固体培养皿表面的接触本身显然是足够的信号。通过改变培养皿表面粘附性，提供了一种非常不同的控制细胞形状的方法。将悬浮的细胞重新铺在预涂有亲水性水凝胶聚（甲基丙烯酸2-羟乙酯）薄层的皿上。然后，最终的平均泡孔直径在宽范围内变化。如前所述，蛋白质合成恢复不受影响。核事件，如DNA和rRNA的合成和mRNA的产生受到细胞形状的深刻影响。细胞表面接触和细胞形状引起明显不同的调节反应。
Anchorage-dependent mouse 3T6 fibroblasts grow only if attached to and spread on a solid substrate. The suspension of cells in methyl-cellulose results in dramatic, coordinated inhibition of the major RNA and protein synthesis systems, and these systems are sequentially restored when cells are replated on a tissue culture dish surface. The effects on metabolism of cell reattachment are separated from those of subsequent spreading by controlling cell shape. Macromolecular metabolism is first strongly suppressed by long-term suspension culture. The cells are then replated in the presence of a variety of spreading inhibitors. The recovery of protein synthesis, which rapidly follows reattachment, does not require extensive cell spreading. Contact of a limited portion of the plasma membrane with the solid culture dish surface is apparently a sufficient signal by itself. A very different method of controlling cell shape is afforded by changing culture dish surface adhesivity. Suspended cells are replated on dishes precoated with thin layers of the hydrophilic hydrogel poly(2-hydroxyethyl methacrylate). The final mean cell diameter is then varied over wide limits. As before, protein synthesis recovery is unaffected. Nuclear events such as DNA and rRNA synthesis and mRNA production are profoundly affected by cell shape. Cell surface contact and cell shape give rise to distinctly different regulatory responses.