The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors
The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors
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DOI：
10.1006/viro.1998.9291
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发表时间：
1998-09-01
期刊：
影响因子：
3.7
通讯作者：
Hughes, SH
中科院分区：
文献类型：
--
作者：
Schaefer-Klein, J;Givol, I;Hughes, SH
缺乏一个良好的永久性，粘附，非转化鸡细胞系，使一些实验与禽白血病肉瘤病毒（ASLV）和载体相当困难。EV-O衍生系DF-I支持亚组（A）、（B）和（C）ASLV以及嗜中性鼠白血病病毒和ASLV衍生载体的有效复制，所述ASLV衍生载体具有源自嗜中性鼠白血病病毒的env基因的env基因。该细胞系适当地响应于转化癌基因（v-myc）至生长抑制基因[p21（wail）]的表达，并且可以通过表达GFP的ASLV载体感染进行分选（使用FAGS）。(C)北京：科学出版社.
The lack of a well-behaved permanent, adherent, nontransformed chicken cell line has made some experiments with avian leukosis-sarcoma viruses (ASLV) and vectors considerably more difficult. The EV-O-derived line, DF-I, supports the efficient replication of subgroups (A), (B), and (C) ASLV, as well as amphotrophic murine leukemia virus and an ASLV-derived vector that has its env gene derived from the env gene from an amphotrophic murine leukemia virus. The cell line responds appropriately to the expression of a transforming oncogene (v-myc) to a growth suppressor gene [p21(wail)] and can be sorted (using FAGS) ii infected by an ASLV vector that expresses GFP. (C) 1998 Academic Press.