Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam
Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam
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DOI：
10.1128/jcm.34.9.2287-2289.1996
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发表时间：
1996-09-01
影响因子：
9.4
通讯作者：
Wataya, W
中科院分区：
文献类型：
--
作者：
Kawamoto, F;Miyake, H;Wataya, W
通过使用吖啶橙子染色法进行快速诊断和使用基于PCR的微量滴定板杂交法进行准确诊断，在越南南部进行了疟疾的现场调查。共检测到3例卵圆疟原虫感染患者，但其中2例患者卵圆疟原虫分离株的PCR扩增DNA与卵圆疟原虫特异性探针不杂交。对18S rRNA基因中靶序列的分析表明，在来自两名患者的分离株的DNA中，来自典型卵形疟原虫序列的探针区域中的三个核苷酸是不同的，其中两个核苷酸缺失，一个核苷酸取代。这些结果可能表明，除了分子生物学方法，仔细的显微镜检查染色的薄血膜仍然需要在不同的疟疾物种的流行率的研究。
Field surveys of malaria were performed in southern Vietnam by using an acridine orange staining method for rapid diagnosis and a PCR-based, microtiter plate hybridization method for accurate diagnosis. A total of three patients of Plasmodium ovale infection were detected, but PCR-amplified DNA of the P. ovale isolates hom two of the patients did not hybridize with the P. ovale-specific probe. Analysis of the target sequence in the 18S rRNA gene indicated that in the DNA of isolates from both patients three nucleotides in the probe region from the typical P. ovale sequence were different, with deletions of two nucleotides and the substitution of one nucleotide. These results may suggest that in addition to molecular biological methods, careful microscopic examination of stained thin blood films is still required in studies of the prevalence of different malaria species.