Mapping of or, a gene conferring orange color on the inner leaf of the Chinese cabbage (Brassica rapa L. ssp pekinensis)
Mapping of or, a gene conferring orange color on the inner leaf of the Chinese cabbage (Brassica rapa L. ssp pekinensis)
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DOI：
10.1007/s11032-010-9542-x
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Liu, Jing
中科院分区：
文献类型：
--
作者：
Feng, Hui;Li, Yuefei;Liu, Jing
大白菜橙色内叶受单隐性基因（或）控制，导致胡萝卜素异常积累。在本研究中，利用一个由600个个体组成的F2群体来定位和开发与该基因密切相关的新标记。从9个细菌人工染色体（BAC）无性系中筛选分布在10个连锁群上的435个SSR标记和16个引物，进行批量分离分析。在连锁分析的基础上，将该基因定位于与大白菜a基因组1号染色体匹配的BAC无性系AC172873和AC189246的2个SSR标记之间总间隔4.6 cM的区域。利用5个SSR标记和2个形态标记构建了该位点的遗传图谱。3个SSR标记与或紧密相连，其中两个SSR标记sau (C) 586和syau19位于同侧，距离分别为1.6和1.3 cM。另一个标记syau15位于另一侧3.3 cM处。通过目测和筛选亲本与两个分离F2群体形态性状的差异，获得了橙色花和橙色子叶（萌发期子叶未变绿前）两个形态标记；两个标记分别为or-f（橙色花）和or-c（橙色子叶）。这两个标记可能与橙内叶性状共分离，或者橙内叶、橙花、橙子叶三个性状是由同一基因决定的。这些标记对大白菜杂交育种的标记辅助选择具有重要意义。
The orange inner leaf of the Chinese cabbage is controlled by a single recessive gene (or), which causes abnormal accumulation of carotene. In the present study, an F2 population consisting of 600 individuals was used for mapping or and developing new markers closely linked to this gene. Bulked segregant analysis was performed by screening 435 simple sequence repeat (SSR) markers well-distributed on 10 linkage groups and 16 SSR primers derived from nine bacterial artificial chromosome (BAC) clones. On the basis of linkage analysis, the or gene was mapped in a region covering a total interval of 4.6 centimorgans (cM) between two SSR markers derived from BAC clones AC172873 and AC189246 at the end of linkage group 9, which matches with chromosome 1 of A genome in Chinese cabbage. A genetic map of the or locus was constructed by using five SSR markers and two morphological markers. Three SSR markers were tightly linked to or and two of them, sau (C) 586 and syau19, were located on the same side at distances of 1.6 and 1.3 cM, respectively. The other marker, syau15, was located on the other side at a distance of 3.3 cM. The two morphological markers, orange flower and orange cotyledon (before cotyledon turns green during the germination period), were obtained by visual determination and screening of the differences in the morphological traits between parents and the two segregated F2 populations; the two markers were designated as or-f (orange flower) and or-c (orange cotyledon). It was suggested that these two markers co-segregate with orange inner leaf trait or that the three characters, namely orange inner leaf, orange flower, and orange cotyledon, are determined by the same gene. These markers could be very helpful for marker-assisted selection in Chinese cabbage hybrid breeding programs.