The sensitivity of Porphyromonas gingivalis and Fusobacterium nucleatum to different (pseudo)halide-peroxidase combinations compared with mutans streptococci
The sensitivity of Porphyromonas gingivalis and Fusobacterium nucleatum to different (pseudo)halide-peroxidase combinations compared with mutans streptococci
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DOI：
10.1099/0022-1317-50-1-42
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发表时间：
2001-01-01
影响因子：
3
通讯作者：
Tenovuo, J
中科院分区：
文献类型：
--
作者：
Ihalin, R;Loimaranta, V;Tenovuo, J
以前的研究表明，过氧化物酶系统与碘化物是特别有效的对放线杆菌伴放线菌。本文研究了碘、氯和硫氰酸盐与乳过氧化物酶（LP）和髓过氧化物酶（MP）联合作用对牙龈卟啉单胞菌、具核梭杆菌、变形链球菌和链球菌活力的影响。分析了大鼠。将细菌在含有过氧化物酶、底物和H2 O2（均为口服生理浓度）的缓冲溶液中孵育，并在0、0.5和1小时后铺板。碘的氧化产物对所有测试的细菌都是最杀菌的。对变形链球菌的作用明显较弱。生理浓度的硫氰酸盐废除的LP-H2 O2-碘化物和MP-H2 O2-碘化物/氯化物组合的影响。硫氰酸盐-过氧化物酶系统已经用于口腔卫生产品。在这些产品中加入碘化物可以使它们对牙周病原体更有效，也有助于防止这些病原体通过唾液在人与人之间传播。
Previous studies have shown that the peroxidase system with iodide is particularly effective against Actinobacillus actinomycetemcomitans. In the present study, the effects of iodide, chloride and thiocyanate in combinations with lactoperoxidase (LP) and myeloperoxidase (MP) on the viability of Porphyromonas gingivalis, Fusobacterium nucleatum, Streptococcus mutans and S. rattus were analysed. Bacteria were incubated in buffer solution containing peroxidase, substrate(s) and H2O2 (all in oral physiological concentrations), and plated after 0, 0.5 and 1 h. The oxidation product of iodide was the most bactericidal against all the bacteria tested. The effect was significantly weaker on mutans streptococci. Physiological concentrations of thiocyanate abolished the effects of LP-H2O2-iodide and MP-H2O2-iodide/chloride combinations. Thiocyanate-peroxidase systems have already been used in oral hygiene products. The incorporation of iodide into these products could make them much more potent against periodontal pathogens, and also help to prevent transmission of these pathogens from person to person via saliva.