Euzebyella marina sp nov., isolated from seawater
Euzebyella marina sp nov., isolated from seawater
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Euzebyella marina sp nov.，从海水中分离
DOI：
10.1099/ijsem.0.001712
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发表时间：
2017
影响因子：
2.8
通讯作者：
Chen Shiyong
中科院分区：
文献类型：
--
作者：
Zhang Yan-Jiao;Zhao Jia-Rui;Zhang Xi-Ying;Chen Guo-Zhong;Zhou Ming-Yang;Mo Xu-Hua;He Hai-Lun;Chen Shiyong
从黄海海水中分离出一种革兰氏染色阴性、好氧、黄色、无鞭毛、非滑动、氧化酶和过氧化氢酶阳性细菌，命名为 CY01T。 CY01T 在 15–37°C（最佳，30°C）、pH 5–8（最佳，6.5–7.5）和 0.5–12 % (w/v) NaCl（最佳，0.5–3.5 %）下生长。它不能产生柔红素型色素或将硝酸盐还原为亚硝酸盐。 CY01T与Euzebyella saccharophila模式菌株的16S rRNA基因序列相似性最高(97.0 %)，并且在16S rRNA基因序列的系统发育树上与Euzebyella属物种紧密聚类。 CY01T 的主要细胞脂肪酸为 iso-C15 : 0、iso-C15 : 1G 和 iso-C17 : 03-OH，主要呼吸醌为甲基萘醌 MK-6。极性脂质包括磷脂酰乙醇胺 (PE)、四种未鉴定的脂质和一种未鉴定的氨基脂质。基因组DNA G+C含量为38.2mol％。基于CY01T的多相表征结果，它代表了Euzebyella属的一个新物种，其名称为Euzebyella marinasp。十一月被提议。模式菌株为CY01T(=CCTCC AB 2014348T=KCTC 42440T)。
A Gram-stain-negative, aerobic, yellow-pigmented, non-flagellated, non-gliding, oxidase- and catalase-positive bacterium, designated CY01T, was isolated from seawater of the Yellow Sea. CY01Tgrew at 15–37 °C (optimum, 30 °C), pH 5–8 (optimum, 6.5–7.5) and with 0.5–12 % (w/v) NaCl (optimum, 0.5–3.5 %). It could not produce flexirubin-type pigment or reduce nitrate to nitrite. CY01Tshowed the highest 16S rRNA gene sequence similarity to the type strain ofEuzebyella saccharophila(97.0 %) and clustered tightly with the species of the genusEuzebyellain the phylogenetic trees based on the 16S rRNA gene sequences. The major cellular fatty acids of CY01Twere iso-C15 : 0, iso-C15 : 1G and iso-C17 : 03-OH and the major respiratory quinone was menaquinone MK-6. Polar lipids included phosphatidylethanolamine (PE), four unidentified lipids and one unidentified aminolipid. The genomic DNA G+C content was 38.2 mol%. Based on the results of the polyphasic characterization of CY01T, it represents a novel species of the genusEuzebyella, for which the nameEuzebyella marinasp. nov. is proposed. The type strain is CY01T(=CCTCC AB 2014348T=KCTC 42440T).