REGULATORY REGION OF MS2-PHAGE RNA REPLICASE CISTRON .4. FUNCTIONAL-ACTIVITY OF SPECIFIC MS2 RNA FRAGMENTS IN FORMATION OF THE 70 S-INITIATION COMPLEX OF PROTEIN-BIOSYNTHESIS
REGULATORY REGION OF MS2-PHAGE RNA REPLICASE CISTRON .4. FUNCTIONAL-ACTIVITY OF SPECIFIC MS2 RNA FRAGMENTS IN FORMATION OF THE 70 S-INITIATION COMPLEX OF PROTEIN-BIOSYNTHESIS
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DOI：
10.1093/nar/6.5.1761
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发表时间：
1979-01-01
影响因子：
14.9
通讯作者：
GREN, EJ
中科院分区：
文献类型：
--
作者：
BORISOVA, GP;VOLKOVA, TM;GREN, EJ
MS2 复制酶顺反子的起始区可以分离为长度为 59 个碱基的片段，通过与充当翻译阻遏物的外壳蛋白的结合来保护免受 RNAase 的影响。该片段 MS2 R(-53—6) 在起始密码子之前 53 个碱基处开始，并保留结合核糖体的全部活性。我们研究了该区域从 5' 端不同程度缩短的一系列片段的起始功能活性。从 AUG 之前 17 或 21 个碱基开始的核糖体保护片段无法与核糖体重新结合。具有此活性的最短片段是通过部分 S1 核酸酶消化产生的，并在 AUG 之前 33 至 35 个碱基处开始。起始信号包含起始密码子前21至33个碱基之间的一些核苷酸，并且负责起始的调节区比最终起始复合物中核糖体保护的调节区长。
The initiation region of the MS2 replicase cistron can be isolated as a fragment 59 bases in lergth protected from RNAase by the binding of the coat protein which serves as a translational repressor. This fragment MS2 R(-53—6) starts 53 bases before the initiation codon and retains full activity in binding ribosomes. We have investigated the functional activity in initiation of a series of fragments from this region variously shortened from the 5′-end. Ribosome protected fragments starting 17 or 21 bases before the AUG are unable to rebind to ribosomes. The shortest fragment which has this activity was produced by partial S1 nuclease digestion and starts 33 to 35 bases before the AUG. The initiation signal comprises some nucleotides between 21 and 33 bases before the initiation codon and the regulatory region responsible for initiation is longer than that protected by the ribosome in the final initiation complex.