Wine polyphenols decrease blood pressure, improve NO vasodilatation, and induce gene expression
Wine polyphenols decrease blood pressure, improve NO vasodilatation, and induce gene expression
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DOI：
10.1161/01.hyp.38.2.159
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发表时间：
2001-08-01
期刊：
影响因子：
8.3
通讯作者：
Andriantsitohaina, R
中科院分区：
文献类型：
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作者：
Diebolt, M;Bucher, B;Andriantsitohaina, R
研究了短期口服红葡萄酒多酚化合物对大鼠血液动力学参数和血管反应性的影响。研究了内皮功能和血管平滑肌收缩性与血管壁中基因表达的诱导的关系。每天通过胃内给予5%葡萄糖或红葡萄酒多酚化合物（20 mg/kg）对大鼠进行治疗，持续7天。这些化合物的给药引起收缩压的进行性降低，在第4天变得显著不同。用红葡萄酒多酚化合物处理的大鼠主动脉显示增加的内皮依赖性舒张乙酰胆碱，这与内皮NO活性增加有关，并涉及对超氧阴离子清除剂敏感的机制。然而，在用红葡萄酒多酚化合物处理的大鼠中没有观察到全身氧化应激的增加，如血浆谷胱甘肽测定所示。此外，在主动脉中，红葡萄酒多酚化合物增加了环氧合酶-2的表达，并增加了内皮血栓素A的释放（2），这补偿了内皮外NO诱导的对去甲肾上腺素的低反应性，这是由诱导型NO合酶表达增强引起的。本研究提供的证据表明，短期口服红葡萄酒多酚化合物产生血压正常大鼠血压下降。这种血流动力学效应与增强的内皮依赖性舒张和动脉壁内基因表达（诱导型NO合酶和环氧合酶-2）的诱导相关，它们共同维持激动剂诱导的收缩性不变。红葡萄酒多酚类化合物的这些作用可能是预防心血管疾病的潜在机制。
The effects of short-term oral administration of red wine polyphenolic compounds on hemodynamic parameters and on vascular reactivity were investigated in rats. Endothelial function and vascular smooth muscle contractility were studied in association with the induction of gene expression in the vascular wall. Rats were treated daily for 7 days by intragastric administration of either 5% glucose or red wine polyphenolic compounds (20 mg/kg). Administration of these compounds produced a progressive decrease in systolic blood pressure, which became significantly different on day 4. Aortas from rats treated with red wine polyphenolic compounds displayed increased endothelium-dependent relaxation to acetylcholine that was related to increased endothelial NO activity and involved a mechanism sensitive to superoxide anion scavengers. However, no increase in whole-body oxidative stress has been observed in rats treated with red wine polyphenolic compounds, as shown by plasma glutathione assay. Also, in the aorta, red wine polyphenolic compounds increased the expression of cyclooxygenase-2 and increased the release of endothelial thromboxane A(2), which compensated for the extraendothelial NO-induced hyporeactivity in response to norepinephrine, resulting from enhanced inducible NO synthase expression. The present study provides evidence that short-term oral administration of red wine polyphenolic compounds produces a decrease in blood pressure in normotensive rats. This hemodynamic effect was associated with an enhanced endothelium-dependent relaxation and an induction of gene expression (of inducible NO synthase and cyclooxygenase-2) within the arterial wall, which together maintain unchanged agonist-induced contractility. These effects of red wine polyphenolic compounds may be a potential mechanism for preventing cardiovascular diseases.