Immobilization of Laccase on Nanoporous Gold: Comparative Studies on the Immobilization Strategies and the Particle Size Effects
Immobilization of Laccase on Nanoporous Gold: Comparative Studies on the Immobilization Strategies and the Particle Size Effects
复制标题
纳米孔金上漆酶的固定化：固定化策略和粒径效应的比较研究
DOI：
10.1021/jp8090304
复制
发表时间：
2009-02-12
影响因子：
3.7
通讯作者：
Gao, Peiji
中科院分区：
文献类型：
--
作者：
Qiu, Huajun;Xu, Caixia;Gao, Peiji
以Au/Ag合金为载体，通过脱银制备纳米多孔金（NPG），并将其用于漆酶的固定化。三种固定策略，即，采用物理吸附、静电吸引和共价偶联等方法对漆酶进行了固定化。从漆酶的负载量、漆酶的比活力和漆酶的渗漏率等方面对三种方法进行了比较。结果表明，物理吸附法是NPG固定化漆酶的最佳方法。这是因为纳米金表面与漆酶残基氨基酸的氨基之间存在潜在的共价键。研究了NPG粒径对漆酶负载量和酶动力学的影响。NPG粒径越小，越有利于漆酶进入内孔固定化。动力学研究表明，破碎的NPG不仅强化了基质及其氧化产物的传质，而且还促进了反应器内的传质。
Nanoporous gold (NPG), prepared simply by dealloying Ag from Au/Ag alloy, was used in the present study as a carrier for laccase immobilization. Three immobilization strategies, i.e., physical adsorption, electrostatic attraction, and covalent coupling, were used to immobilize laccase on NPG. A detailed comparison among the three strategies was made in light of the loading, the specific activity, and the leakage of laccase. The present results indicated that the physical adsorption strategy was the best one for laccase immobilization on NPG. This was because of the potential covalent linkage between the nanoscale gold surface and the amino groups of the residue amino acids of laccase. The effects of the particle size of NPG on laccase loading and enzyme kinetics were also investigated. When the particle size of NPG got smaller, more laccase could access the inner pore and be immobilized. The kinetic study showed that the crushed NPG not only enhanced mass transfer of the substrate and its oxidation produc...