Kinesin-dependent axonal transport is mediated by the Sunday driver (SYD) protein
Kinesin-dependent axonal transport is mediated by the Sunday driver (SYD) protein
复制标题
DOI：
10.1016/s0092-8674(00)00162-8
复制
发表时间：
2000-11-10
期刊：
影响因子：
64.5
通讯作者：
Goldstein, LSB
中科院分区：
文献类型：
--
作者：
Bowman, AB;Kamal, A;Goldstein, LSB
鉴定了一种广泛保守的膜相关蛋白，它是驱动蛋白-1与轴突货物功能相互作用所必需的。SUNDAY DIVER(Syd)和轴突运输运动蛋白Kinesin-1的突变会导致果蝇类似的表型，包括轴突货物的异常堆积。GFP标记的哺乳动物SYD定位于肾小管上皮细胞结构，该结构结合了Kinesin-1和分泌途径的标志物。免疫共沉淀分析表明，小鼠SYD在体内与Kinesin-1形成了络合物。酵母双杂交分析和体外相互作用研究表明，SYD通过Kinesin轻链(KLC)的四肽重复(TPR)结构域直接与Kinesin-1结合，K-d约为200 nM。我们认为SYD通过直接与KLC相互作用来调节至少一类囊泡的轴突运输。
A broadly conserved membrane-associated protein required for the functional interaction of kinesin-1 with axonal cargo was identified. Mutations in sunday driver (syd) and the axonal transport motor kinesin-1 cause similar phenotypes in Drosophila, including aberrant accumulations of axonal cargoes. GFP-tagged mammalian SYD localizes to tubulovesicular structures that costain for kinesin-1 and a marker of the secretory pathway. Coimmunoprecipitation analysis indicates that mouse SYD forms a complex with kinesin-1 in vivo. Yeast two-hybrid analysis and in vitro interaction studies reveal that SYD directly binds kinesin-1 via the tetratricopeptide repeat (TPR) domain of kinesin light chain (KLC) with K-d approximate to 200 nM. We propose that SYD mediates the axonal transport of at least one class of vesicles by interacting directly with KLC.