Primary central nervous system lymphomas in immunocompetent individuals: Histology, Epstein-Barr virus genome, Ki-67 proliferation index, p53 and bcl-2 gene expression
Primary central nervous system lymphomas in immunocompetent individuals: Histology, Epstein-Barr virus genome, Ki-67 proliferation index, p53 and bcl-2 gene expression
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DOI：
10.3109/10428199809050936
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发表时间：
1998-06-01
影响因子：
2.6
通讯作者：
D'Amore, F
中科院分区：
文献类型：
--
作者：
Krogh-Jensen, M;Johansen, P;D'Amore, F
在绝大多数HN相关的原发性中枢神经系统淋巴瘤（PCNSL）中检测到EB病毒（EBV），表明该病毒的致病作用。与HIV相关PCNSL不同，关于非免疫缺陷相关（散发性）PCNSL中EBV的存在相互矛盾的数据：因此，使用RNA原位杂交（RISH）分析了41例散发性PCNSL的基于人群的材料中EBV基因组（EBER，BHLF）的存在。此外，bcl-2癌基因和p53肿瘤抑制基因的基因产物的表达和与单克隆抗体Ki-67反应的肿瘤生长分数已被评估。除两例外，所有病例均为EBV基因组阴性。在两例阳性病例中，不到5%的肿瘤细胞显示EBER阳性。相比之下，在两例HIV相关PCNSL中，超过75%的细胞形态学上属于肿瘤细胞群，EBER染色阳性。bcl-2癌蛋白免疫组化染色阳性28例（72%）。在大多数情况下，超过75%的肿瘤细胞显示胞质表达。在37例p53表达的病例中，21例（57%）阳性。然而，在绝大多数阳性病例中，少于10%的肿瘤细胞染色。Ki-67阳性细胞的百分比范围为10%至80%，平均为50%。p53和bcl-2癌蛋白和生长分数的表达没有任何预后的影响。我们得出结论，EBV基因组很少检测到散发性PCNSL，表明EBV的致病作用是不可能的。与脑外B细胞淋巴瘤一样，大部分PCNSL表达p53和bcl-2癌蛋白，然而，这一特征似乎并不具有预后意义。
Epstein-Barr virus (EBV) has been detected in the large majority of HN-related primary central nervous system lymphomas (PCNSL) suggesting a pathogenetic role of the virus. Unlike HIV-related PCNSL, conflicting data exist with regard to the presence of EBV in non immunodeficiency-related (sporadic) PCNSL: For this reason, a population based material of 41 sporadic PCNSL was analysed for the presence of EBV genome (EBER, BHLF) using RNA in situ hybridisation (RISH). Furthermore, the expression of the gene products of the bcl-2 oncogene and the p53 tumor suppressor gene and the tumor growth fraction reactive with the monoclonal antibody Ki-67 have been evaluated. All cases but two were EBV genome negative. In the two positive cases less than 5% of tumor cells showed EBER positivity. In contrast, more than 75% of cells morphologically belonging to the tumor-cell population stained positively for EBER in two cases of HIV related PCNSL. Immunostaining for the bcl-2 oncoprotein was positive in 28 (72%) of 39 cases examined. In most cases more than 75% of tumor cells showed cytoplasmic expression. Of 37 cases investigated for p53 expression, 21 (57%) stained positively. However, in the large majority of positive cases less than 10% of the neoplastic cells stained. The percentage of Ki-67 positive cells ranged between 10% and 80% with a mean of 50%. The expression of the p53 and bcl-2 oncoproteins and the growth fraction did not have any prognostic impact.We conclude that the EBV genome is rarely detected in sporadic PCNSL, indicating that a pathogenetic role of EBV is unlikely. Like extracerebral B-cell lymphomas a large fraction of PCNSL expresses the p53 and bcl-2 oncoproteins, a feature, however, which does not seem to have prognostic implications.