Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors
Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors
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与白色和黑色毛色相关的绵羊皮肤中血管紧张素II的表达和组织分布分析
DOI：
10.1016/j.acthis.2019.03.002
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发表时间：
2019-01-01
影响因子：
2.5
通讯作者：
Liu, Xingyou
中科院分区：
文献类型：
--
作者：
Yin, Zhihong;Ge, Yaming;Liu, Xingyou
血管紧张素II（AngII）通过酪氨酸酶调节黑素细胞中的色素合成。为探讨AngII与毛色形成的相关性，采用液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）、Western blot、实时荧光定量PCR（qPCR）和免疫组织化学方法检测AngII在绵羊白色和黑色皮肤组织中的表达分布，结果表明AngII在绵羊白色和黑色皮肤组织中均存在。Western blot结果验证了LC-ESI-MS/MS结果，表明AngII在黑绵羊皮肤中的表达水平显著高于白色绵羊皮肤。定量真实的时间PCR（qRT-PCR）结果还显示，AngII mRNA在黑绵羊皮肤中的表达水平高于白色绵羊皮肤。免疫组织化学分析进一步证实AngII蛋白定位于绵羊毛囊的毛球和外根鞘。总之，蛋白质和转录本在白色和黑色绵羊皮中表现出相同的表达模式。AngII在黑羊的毛球和外根鞘中的表达强于白色羊。这些结果表明AngII在绵羊毛色调控中发挥重要作用，为进一步研究AngII在绵羊毛色形成中的作用提供了新的思路。
Angiotensin II (AngII) regulates pigment synthesis by tyrosinase in melanocytes. To evaluate the association between AngII and coat color formation, we detected the expression distribution of AngII in white and black sheep skins by LC-ESI-MS/MS, western blot, quantitative real-time-PCR (qPCR) and distribution of AngII by immunohistochemistry.Liquid chromatography-electrospray ionization tandem MS (LC-ESI-MS/MS) results showed that AngII was found in white and black skin tissues of sheep. Western blot results verified the LC-ESI-MS/MS results and suggested that AngII was expressed at significantly higher levels in black sheep skins compared with the white sheep skins. Quantitative real time PCR (qRT-PCR) results also revealed that the expression level of AngII mRNA was higher in black sheep skins than that in white sheep skins. Immunohistochemical analysis further demonstrated that AngII protein was localized in the hair bulb and outer root sheath of hair follicle in sheep. In summary, protein and transcripts exhibited the same expression pattern in white and black sheep skins. Furthermore, the expressions of AngII in the hair bulb and outer root sheath of black sheep were stronger than those in white sheep. These results suggested that AngII functions in sheep coat color regulation and offer a novel insight for further investigation on the role of AngII in the coat color formation in sheep.