Open‐circuit sodium and chloride fluxes across isolated opercular epithelia from the teleost Fundulus heteroclitus.
Open‐circuit sodium and chloride fluxes across isolated opercular epithelia from the teleost Fundulus heteroclitus.
复制标题
开路钠和氯化物穿过来自硬骨鱼眼底异斜的孤立的鳃盖上皮细胞。
DOI：
10.1113/jphysiol.1979.sp012942
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
J. Zadunaisky
中科院分区：
文献类型：
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作者：
K. Degnan;J. Zadunaisky
1. 在开路条件下，在用海水沐浴粘膜和用林格溶液沐浴浆膜时，在体外测量海水适应的异斜眼底上皮细胞的 Na+ 和 Cl- 通量。 2. 平均预测 Na+ 通量比率为 0.94 +/- 0.08，观测比率为 1.14 +/- 0.12（n = 15；平均值 +/- S.E.）。这些平均值的差异不显着（P 大于 0.20）。平均预测 Cl 通量比率为 11.4 +/- 0.9，平均观测比率为 1.38 +/- 0.27 (n = 10)。这些平均值的差异显着（P 小于 0.001）。 3. 哇巴因在浆膜溶液中浓度为 10(‐6) M 时，可显着（P 小于 0.01）减少 Na+ 流出，但对 Na+ 流入没有显着（P 大于 0.40）影响。哇巴因治疗后预测的 (1.70 +/- 0.14) 和观察到的 (1.72 +/- 0.18) Na+ 通量比率之间的一致性表明，这种效应完全归因于 ATP 酶抑制继发的上皮去极化。 4.异丙肾上腺素激活β-肾上腺素刺激Cl-流出24.2%，去甲肾上腺素激活α-肾上腺素抑制Cl-流出66.5%。这些变化的发生与这些物质产生的电梯度变化所预测的相反，而 Cl-流入的变化与电变化相对应。短路实验证实了这些对 Cl- 流出的影响，而对 Cl- 流入没有影响。 5. 结果表明Na+接近热力学平衡并且单向通量是被动的。 α-和β-肾上腺素能激活的作用表明，活性Cl-分泌可能受到儿茶酚胺的拮抗调节。
1. The Na+ and Cl‐ fluxes across opercular epithelia from sea water‐adapted Fundulus heteroclitus were measured in vitro under open‐circuit conditions while bathed on the mucosa with sea water and the serosa with Ringer solution. 2. The mean predicted Na+ flux ratio was 0.94 +/‐ 0.08 and the observed ratio was 1.14 +/‐ 0.12 (n = 15; mean +/‐ S.E. of mean). The difference in these means was not significant (P greater than 0.20). The mean predicted Cl‐ flux ratio was 11.4 +/‐ 0.9 and the mean observed ratio was 1.38 +/‐ 0.27 (n = 10). The difference in these means was significant (P less than 0.001). 3. Ouabain, at 10(‐6) M in the serosal solution, produced a significant (P less than 0.01) reduction in the Na+ efflux while having no significant (P greater than 0.40) effect on the Na+ influx. The agreement between the predicted (1.70 +/‐ 0.14) and observed (1.72 +/‐ 0.18) Na+ flux ratios after ouabain treatment suggested that this effect could be completely attributed to the depolarization of the epithelium secondary to ATPase inhibition. 4. beta‐adrenergic activation by isoprenaline stimulated the Cl‐ efflux 24.2% and alpha‐adrenergic activation by noradrenaline inhibited the Cl‐ efflux 66.5%. These changes occurred oppositely to those predicted by the changes in the electrical gradient produced by these agents, while the changes in the Cl‐ influxes corresponded to the electrical changes. Short‐circuit experiments confirmed these effects on the Cl‐ efflux and the lack of effects on the Cl‐ influx. 5. The results suggested that Na+ was near theromodynamic equilibrium and that the unidirectional fluxes were passive. The effects of alpha‐ and beta‐adrenergic activation suggested that the active Cl‐ secretion may be antagonistically regulated by catecholamines.