REVERSAL OF X-INACTIVATION IN FEMALE MOUSE SOMATIC-CELLS HYBRIDIZED WITH MURINE TERATOCARCINOMA STEM-CELLS INVITRO
REVERSAL OF X-INACTIVATION IN FEMALE MOUSE SOMATIC-CELLS HYBRIDIZED WITH MURINE TERATOCARCINOMA STEM-CELLS INVITRO
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DOI：
10.1016/0092-8674(83)90563-9
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
SASAKI, M
中科院分区：
文献类型：
--
作者：
TAKAGI, N;YOSHIDA, MA;SASAKI, M
用聚乙二醇介导法将具有1条X染色体的小鼠胚胎癌细胞(PSA-6TG1或OTF9-63)与携带Cattanach‘S或Searle’S易位的雌性小鼠的胸腺或骨髓细胞融合，获得一系列近二倍体胚胎癌[EC]样杂交细胞。在与EC细胞融合之前，体细胞被认为只含有1条活性X染色体。杂种形成后，X染色体复制年表和X连锁基因pGK-1的表达表明，双亲贡献的所有X染色体在这些杂交种中都是活跃的。实验排除了EC细胞与罕见的X染色体都活跃的体细胞融合形成杂交细胞的可能性。在融合的4天内，整个不活跃的X染色体都会重新激活。
A series of near-diploid embryonal carcinoma[EC]-like hybrid cells were obtained from polyethylene glycol-mediated cell fusion between murine embryonal carcinoma cells (PSA-6TG1 or OTF9-63) having 1 X chromosome and thymocytes or bone marrow cells from female mice carrying Cattanach''s or Searle''s translocation. Prior to fusion with EC cells the somatic cells are presumed to contain only 1 active X chromosome. Following hybrid formation, the chronology of X chromosome replication and the expression of X-linked gene Pgk-1 indicated that all X chromosomes contributed by both parents were active in these hybrids. Experiments were performed to rule out the possibility that the hybrids were formed by fusion of EC cells with rare somatic cells in which both X chromosomes were active. Within 4 days of fusion there is reactivation of the entire inactive X chromosome.