The protective role of silicon in the Arabidopsis-powdery mildew pathosystem
The protective role of silicon in the Arabidopsis-powdery mildew pathosystem
复制标题
DOI：
10.1073/pnas.0606330103
复制
发表时间：
2006-11-14
影响因子：
11.1
通讯作者：
Belanger, Richard R.
中科院分区：
文献类型：
--
作者：
Fauteux, Francois;Chain, Florian;Belanger, Richard R.
硅（Si）在植物生物学中的作用和重要性已经争论了超过150年，尽管许多报道描述了其有益特性。为了获得关于Si对植物的影响的独特见解，我们使用44 K微阵列对对照和白粉病胁迫的拟南芥植物（有或没有Si应用）进行了完整的转录组分析。令人惊讶的是，在对照植物中，除了两个基因外，所有基因的表达都不受Si的影响，这一结果与Si作为肥料可能直接影响的报道相矛盾。相比之下，接种植物，无论是否用硅处理，都改变了一组近4,000个基因的表达。功能分类后，许多上调的基因是防御相关的，而大部分下调的基因参与初级代谢。受调控的防御基因包括R基因、逆境相关转录因子、信号转导基因、逆境激素（SA、JA、乙烯）的生物合成基因和活性氧代谢基因。在用Si处理的接种植物中，下调的幅度减弱> 25%，这是胁迫缓解的指示。我们的研究结果表明，硅处理对非胁迫植物的代谢没有影响，这表明元素的非必要作用，但它具有有益的特性，可归因于调制更有效的响应病原体胁迫。
The role and essentiality of silicon (Si) in plant biology have been debated for > 150 years despite numerous reports describing its beneficial properties. To obtain unique insights regarding the effect of Si on plants, we performed a complete transcriptome analysis of both control and powdery mildew-stressed Arabidopsis plants, with or without Si application, using a 44K microarray. Surprisingly, the expression of all but two genes was unaffected by Si in control plants, a result contradicting reports of a possible direct effect of Si as a fertilizer. In contrast, inoculation of plants, treated or not with Si, altered the expression of a set of nearly 4,000 genes. After functional categorization, many of the up-regulated genes were defense-related, whereas a large proportion of down-regulated genes were involved in primary metabolism. Regulated defense genes included R genes, stress-related transcription factors, genes involved in signal transduction, the biosynthesis of stress hormones (SA, JA, ethylene), and the metabolism of reactive oxygen species. In inoculated plants treated with Si, the magnitude of down-regulation was attenuated by > 25%, an indication of stress alleviation. Our results demonstrate that Si treatment had no effect on the metabolism of unstressed plants, suggesting a nonessential role for the element but that it has beneficial properties attributable to modulation of a more efficient response to pathogen stress.