High hydrostatic pressure and enzymic activity: inhibition of multimeric enzymes by dissociation.
High hydrostatic pressure and enzymic activity: inhibition of multimeric enzymes by dissociation.
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高静水压和酶活性：通过解离抑制多聚酶。
DOI：
10.1016/0003-9861(71)90290-6
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发表时间：
1971
影响因子：
3.9
通讯作者：
J. T. Penniston
中科院分区：
文献类型：
--
作者：
J. T. Penniston
当采取预防措施使酶与其底物饱和并防止压力变性时，发现多聚体酶的活性被抑制，而单体酶的活性被高静水压的应用所刺激。被抑制的多聚体酶是线粒体ATP酶（通过ATP水解或ATP-32 Pi交换活性测量）、红细胞膜ATP酶、肌管囊泡ATP酶、丙酮酸羧化酶、乙酰氨基琥珀酸酶、碱性磷酸酶和肌酸激酶。被刺激的单体酶有过氧化物酶、肌激酶、胰蛋白酶、淀粉酶、溶菌酶和胰凝乳蛋白酶。理论上的考虑和先前工作者的实验研究结果都表明，高静水压力将使通过非共价相互作用结合在一起的蛋白质多聚体解离。这种解离必须被认为是压力对酶系统影响的主要决定因素，因为酶系统的活性依赖于这种蛋白质-蛋白质相互作用。由于多聚体酶的类别包括一个大比例的已知酶，解聚作用必须被认为是一个主要因素，在调查压力对所有细胞系统的影响。这一结论与深海生物的研究特别相关。它们的酶系统要么必须是单体的，要么必须在多聚体之间具有更强的非共价相互作用，以便在这些生物体生存的高压下运行。
When precautions were taken to saturate enzymes with their substrates and to prevent pressure denaturation, it was found that the activities of multimeric enzymes were inhibited and the activities of monomeric enzymes stimulated by application of high hydrostatic pressure. Multimeric enzymes which were inhibited were mitochondrial ATPase (measured by hydrolysis of ATP or by ATP-32Piexchange activity), erythrocyte membrane ATPase, sarcotubular vesicle ATPase, pyruvate carboxylase, argininosuccinase, alkaline phosphatase, and creatine kinase. Monomeric enzymes which were stimulated were peroxidase, myokinase, trypsin, amylase, lysozyme and chymotrypsin. Both theoretical considerations and the results of experimental studies by previous workers show that high hydrostatic pressure will dissociate protein multimers which are held together by noncovalent interactions. This dissociation must be considered as the major determinant of the effect of pressure on enzymic systems which depend upon such protein-protein interactions for their activity. Since the category of multimeric enzymes includes a large proportion of known enzymes, dissaggregating effects must be considered as a major factor in investigating the effects of pressure on all cellular systems. This conclusion is particularly relevant to the study of deep-sea organisms. Their enzyme systems either must be monomeric, or must have stronger noncovalent interactions between multimers, in order to operate at the high pressures under which these organisms live.