Optimization of Dilution and Mixing of Biochemical Samples Using Digital Microfluidic Biochips
Optimization of Dilution and Mixing of Biochemical Samples Using Digital Microfluidic Biochips
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DOI：
10.1109/tcad.2010.2061790
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发表时间：
2010-11
影响因子：
2.9
通讯作者：
Sudip Roy;B. Bhattacharya;K. Chakrabarty
中科院分区：
文献类型：
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作者：
Sudip Roy;B. Bhattacharya;K. Chakrabarty
最近的实验室芯片（LOC）技术的出现导致许多与医疗保健相关的应用领域（例如，护理点临床诊断，高通量测序和蛋白质组学）的范式转移。有希望的LOC类别是基于数字微流体（DMF）的生物芯片，其中纳米级体积流体液滴在2D电极阵列上操纵。将此类芯片和映射实验室基础协议映射到LOC的关键挑战是有效地执行生化样品的稀释过程。作为一种优化和自动化技术，我们提出了一种稀释/混合算法，可显着减少废物液滴的产生。该算法需要O（n）时间来计算最多需要n顺序混合/拆分操作，以达到任何给定的目标浓度，而浓度因子的误差小于[1/（2n）]。为了实现算法，我们设计了一个基于DMF的LOC的架构布局，该布局由两个O（n）大小的旋转混合器和O（n）储物电极组成。仿真结果表明，与早期方法相比，该提出的技术始终可以在废液的数量中节省非负节省，并且在输入液滴总数中也可以节省。
The recent emergence of lab-on-a-chip (LoC) technology has led to a paradigm shift in many healthcare-related application areas, e.g., point-of-care clinical diagnostics, high-throughput sequencing, and proteomics. A promising category of LoCs is digital microfluidic (DMF)-based biochips, in which nanoliter-volume fluid droplets are manipulated on a 2-D electrode array. A key challenge in designing such chips and mapping lab-bench protocols to a LoC is to carry out the dilution process of biochemical samples efficiently. As an optimization and automation technique, we present a dilution/mixing algorithm that significantly reduces the production of waste droplets. This algorithm takes O(n) time to compute at most n sequential mix/split operations required to achieve any given target concentration with an error in concentration factor less than [1/(2n)]. To implement the algorithm, we design an architectural layout of a DMF-based LoC consisting of two O(n)-size rotary mixers and O(n) storage electrodes. Simulation results show that the proposed technique always yields nonnegative savings in the number of waste droplets and also in the total number of input droplets compared to earlier methods.