Glucose and lactate biosensors prepared by a layer-by-layer deposition of concanavalin A and mannose-labeled enzymes: electrochemical response in the presence of electron mediators.
Glucose and lactate biosensors prepared by a layer-by-layer deposition of concanavalin A and mannose-labeled enzymes: electrochemical response in the presence of electron mediators.
复制标题
通过逐层沉积伴刀豆球蛋白 A 和甘露糖标记酶制备的葡萄糖和乳酸生物传感器：电子介体存在下的电化学响应。
DOI：
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发表时间：
2001
影响因子：
1.7
通讯作者：
J. Anzai
中科院分区：
文献类型：
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作者：
Y. Kobayashi;T. Hoshi;J. Anzai
以葡萄糖氧化酶（GOx）和乳酸氧化酶（LOx）为修饰电极，以二茂铁衍生物为电子介质，研究了葡萄糖和乳酸生物传感器的电化学响应。在循环伏安法中，葡萄糖和乳酸生物传感器分别显示出对葡萄糖和乳酸的催化电流，这表明二茂铁衍生物可以介导电子从薄膜中的还原形式的GOx和LOx顺利地传递到电极。在所用的三种二茂铁衍生物中，二茂铁甲醇因其氧化电位低而被认为是最合适的电子介体。葡萄糖和乳酸传感器给出了有用的校准图，其中达到更高的检测限相比，当传感器在没有电子介体的情况下操作时所观察到的。
An electrochemical response of glucose and lactate biosensors which were prepared by coating a platinum electrode with a thin film composed of concanavalin A and mannose-labeled glucose oxidase (GOx) or lactate oxidase (LOx) was evaluated in the presence of ferrocene derivatives as electron mediator. Both glucose and lactate biosensors showed catalytic current to glucose and lactate, respectively, in cyclic voltammetry, suggesting that the ferrocene derivatives can mediate electron transport smoothly from the reduced forms of GOx and LOx in the thin films to the electrode. Among the three kinds of ferrocene derivatives used, ferrocenylmethanol was found to be the most suitable electron mediator because of its low oxidation potential. The glucose and lactate sensors gave useful calibration graphs, in which higher detection limits were reached as compared with those observed when the sensors were operated in the absence of electron mediator.