Positive correlation between pregnancy-associated plasma protein-A level and OX40 ligand expression in patients with acute coronary syndromes
Positive correlation between pregnancy-associated plasma protein-A level and OX40 ligand expression in patients with acute coronary syndromes
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DOI：
10.1016/j.biopha.2010.10.011
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发表时间：
2011-06-01
影响因子：
7.5
通讯作者：
Chen, Guanghua
中科院分区：
文献类型：
--
作者：
Liu, Peijing;Yan, Jinchuan;Chen, Guanghua
目标。-越来越多的证据表明，血清妊娠相关血浆蛋白-A(PAPP-A)和OX40配体(OX40L)的表达与急性冠脉综合征(ACS)有关。探讨急性冠脉综合征患者血清PAPP-A水平与OX40L及膜结合型OX40L的关系。-本研究包括正常对照组(n=30)、稳定型心绞痛(SA)患者(n=60)和急性冠脉综合征(ACS)患者，包括不稳定型心绞痛(UA)(n=50)和急性心肌梗死(AMI)(n=30)。用双抗体夹心法测定血清PAPP-A和可溶性OX40L(SOX40L)的浓度，用流式细胞术分析单核细胞表面OX40L的表达。UA组[(25.6±5.5)MFI]和AMI组(29.4+/-6.3MFI)外周血单核细胞OX40L的表达明显高于SA组(10.6+/-2.8MFI)和对照组(11.1+/-3.5MFI)。UA组sOX40L、PAPP-A分别为15.7+/-4.9、25.4+/-6.8 mg/mL，急性心肌梗死组sOX40L、PAPP-A分别为17.1+/-5.3、26.3+/-5.6mU/mL，均显著高于SA组(3.4+/-1.4、9.6+/-2.1mU/mL)和正常对照组(3.9+/-1.3 ng/mL)。8.5+/-2.8微克/毫升)(P<；0.001)。有趣的是，sOX40L、膜结合型OX40L与血清PAPP-A水平呈正相关(r(1)=0.54，r(2)=0.51；P<；0.0001)。血清和膜结合型OX40L和PAPP-A水平与复杂冠状动脉狭窄显著相关(r(1)=0.56，r(2)=0.55，r(3)=0.40；P<；0.001)。-PAPP-A水平与急性冠脉综合征患者的可溶性和膜结合型OX40L显著相关。血清PAPP-A和sOX40L水平升高可能是冠心病活动性的一个预后指标。(C)2010年爱思唯尔·马森公司。版权所有。
Objective. - Increasing evidence show that serum pregnancy-associated plasma protein-A (PAPP-A) and OX40 ligand (OX40L) expression have been implicated in acute coronary syndromes (ACS). We investigated the relationship between PAPP-A level and OX40L in serum and membrane-bound OX40L in patients with ACS.Methods. - The present study included normal controls (n = 30), patients with stable angina (SA) (n = 60) and patients with ACS, including unstable angina (UA) (n = 50) and acute myocardial infarction (AMI) (n = 30). Serum concentrations of PAPP-A and soluble OX40L (sOX40L) were determined with Elisa, whereas the expression of OX40L on monocytes were analyzed with flow cytometry.Results. -The expression of OX40L in peripheral monocytes in patients with UA [25.6 + 5.5 mean fluorescence intensity (MFI)] and AMI (29.4 +/- 6.3 MFI) were significantly higher than those in patients with SA (10.6 +/- 2.8 MFI) and controls (11.1 +/- 3.5 MFI). Both sOX40L and PAPP-A in patients with UA (15.7 +/- 4.9 ng/ mL, 25.4 +/- 6.8 mg/mL, respectively) and AMI (17.1 +/- 5.3 ng/ mL, 26.3 +/- 5.6 mu g/mL, respectively) were significantly higher than those in patients with SA (3.4 +/- 1.4 ng/mL, 9.6 +/- 2.1 mu g/ mL, respectively) and controls (3.9 +/- 1.3 ng/mL, 8.5 +/- 2.8 mu g/mL, respectively) (P < 0.001). Interestingly, a positive correlation was found between sOX40L, membrane-bound OX40L and serum PAPP-A levels (r(1) = 0.54, r(2) = 0.51; P < 0.0001). Both serum and membrane-bound OX40L and PAPP-A levels significantly correlated with complex coronary stenosis (r(1) = 0.56, r(2) = 0.55, r(3) = 0.40; P < 0.001).Conclusion. - PAPP-A level was significantly related to soluble and membrane-bound OX40L in patients with ACS. Enhanced level of serum PAPP-A and sOX40L might represent a prognostic marker for coronary disease activity. (C) 2010 Elsevier Masson SAS. All rights reserved.