Glycomic studies of Drosophila melanogaster embryos.
Glycomic studies of Drosophila melanogaster embryos.
复制标题
果蝇胚胎的糖组研究。
DOI：
10.1007/s10719-006-6693-4
复制
发表时间：
2006
影响因子：
3
通讯作者：
Haslam,StuartM
中科院分区：
文献类型：
--
作者：
North,SimonJ;Koles,Kate;Hembd,Caleb;Morris,HowardR;Dell,Anne;Panin,VladislavM;Haslam,StuartM
随着黑腹果蝇全基因组序列的确定，一种理解糖基化功能的系统方法已经成为可能。整个果蝇糖蛋白在特定发育阶段的结构分配可以提供信息，进一步阐明不同聚糖在发育过程中的具体作用，并查明某些糖基转移酶和其他聚糖生物合成基因的活性，否则可能会通过遗传分析错过这些基因。在这篇论文中，我们描述了果蝇胚胎的主要糖蛋白N-和o -聚糖，作为我们对黑腹果蝇糖蛋白特征的初步研究的一部分。n -聚糖以高甘露糖和低甘露糖结构为主。在GlcNAc和Galβ1-4GlcNAc非还原末端中观察到少量的单、双和三天线复合聚糖。o -聚糖仅限于粘蛋白型核心1 Galβ1-3GalNAc序列。
With the complete genome sequence ofDrosophila melanogasterdefined a systematic approach towards understanding the function of glycosylation has become possible. Structural assignment of the entireDrosophilaglycome during specific developmental stages could provide information that would shed further light on the specific roles of different glycans during development and pinpoint the activity of certain glycosyltransferases and other glycan biosynthetic genes that otherwise might be missed through genetic analyses. In this paper the major glycoprotein N- and O-glycans ofDrosophilaembryos are described as part of our initial undertaking to characterize the glycome ofDrosophila melanogaster. The N-glycans are dominated by high mannose and paucimannose structures. Minor amounts of mono-, bi- and tri-antennary complex glycans were observed with GlcNAc and Galβ1–4GlcNAc non-reducing end termini. O-glycans were restricted to the mucin-type core 1 Galβ1-3GalNAc sequence.