Transgenic rice plants expressing human CYP1A1 remediate the triazine herbicides atrazine and simazine
Transgenic rice plants expressing human CYP1A1 remediate the triazine herbicides atrazine and simazine
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DOI：
10.1021/jf051370f
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发表时间：
2005-11-02
影响因子：
6.1
通讯作者：
Ohkawa, Y
中科院分区：
文献类型：
--
作者：
Kawahigashi, H;Hirose, S;Ohkawa, Y
将人细胞色素P450 CYP 1A 1基因导入水稻（Oryza sativa cv. Nipponbare）。在土壤中生长的一个月大的CYP 1A 1植物清楚地显示出健康的生长和对8.8 μ M阿特拉津和50 μ M西玛津的耐受性，但非转基因植物被除草剂完全杀死。虽然转基因和非转基因植物代谢的两种除草剂成相同的化合物，CYP 1A 1植物代谢阿特拉津和西玛津比对照植物更迅速。在小规模试验中，CYP 1A 1植物培养基中阿特拉津和西玛津的残留量分别为对照培养基中的43.4%和12.3%;非转基因日本晴分别为68.3%和57.2%。当在含有2.95 μ M阿特拉津和3.15 μ M西玛津的土壤中培养25天时，CYP 1A 1植物从土壤中消除的阿特拉津和西玛津分别是对照植物的1.3倍和1.4倍。因此，CYP 1A 1水稻植株可以比非转基因日本晴更快地从培养基和土壤中去除阿特拉津和西玛津。
The human cytochrome P450 CYP1A1 gene was introduced into rice plants (Oryza sativa cv. Nipponbare). One-month-old CYP1A1 plants grown in soil clearly showed a healthy growth and tolerance to 8.8 mu M atrazine and 50 mu M simazine, but nontransgenic plants were completely killed by the herbicides. Although transgenic and nontransgenic plants metabolized the two herbicides into the same sets of compounds, CYP1A1 plants metabolized atrazine and simazine more rapidly than did control plants. In small-scale experiments, residual amounts of atrazine and simazine in the culture medium of CYP1A1 plants were 43.4 and 12.3% of those in control medium; those of nontransgenic Nipponbare were 68.3 and 57.2%, respectively. When cultivated in soil with 2.95 mu M atrazine and 3.15 mu M simazine for 25 days, CYP1A1 plants eliminated 1.3 times more atrazine and 1.4 times more simazine from the soil than did control plants. Thus, CYP1A1 rice plants make it possible to remove atrazine and simazine more rapidly from the culture medium and soil than can nontransgenic Nipponbare.