Frequent hypomethylation in Wilms tumors of pericentromeric DNA in chromosomes 1 and 16
Frequent hypomethylation in Wilms tumors of pericentromeric DNA in chromosomes 1 and 16
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DOI：
10.1016/s0165-4608(98)00143-5
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发表时间：
1999-02-01
影响因子：
--
通讯作者：
Ehrlich, M
中科院分区：
文献类型：
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作者：
Qu, GZ;Grundy, PE;Ehrlich, M
1号或16号染色体的近中心异染色质重排在许多类型的癌症中经常发现，包括Wilms肿瘤，并且被认为有助于肿瘤的发生或肿瘤的进展。这些重排的致癌潜力归因于影响肿瘤抑制基因或原癌基因剂量的染色体臂失衡。由于DNA低甲基化与两种非癌细胞群体中1号染色体和16号染色体周围中心区域的重排有关，因此我们研究了Wilms肿瘤中这些区域正常高度甲基化的卫星DNA序列的甲基化。低甲基化在染色体近中心粒（卫星2）序列中很常见，尤其是在1号染色体的着丝粒（卫星α）序列中。16号染色体卫星2 DNA的低甲基化与1号染色体卫星2 DNA的低甲基化高度一致。我们讨论了Wilms肿瘤中这种卫星DNA低甲基化与染色体畸变的关系，通过测定杂合性的丧失来确定。(C) Elsevier Science Inc., 1999。版权所有。
Rearrangements in the pericentromeric heterochromatin of chromosome 1 or 16 are often found in many types of cancers, including Wilms tumors, and have been suggested to contribute to oncogenesis or tumor progression. The oncogenic potential of these rearrangements has been ascribed to the resulting chromosome arm imbalances affecting the dosage of tumor suppressor genes or protooncogenes. Because DNA hypomethylation has been linked to rearrangements in the pericentromeric regions of chromosome 1 and 16 in two types of non-cancer cell populations, we examined methylation of normally highly methylated satellite DNA sequences in these regions in Wilms tumors. Hypomethylation was found to be frequent in juxtacentromeric (satellite 2) sequences and, especially, in centromeric (satellite alpha) sequences of chromosome 1. Hypomethylation of satellite 2 DNA of chromosome 16 showed a high degree of concordance with that of satellite 2 DNA of chromosome 1. We discuss the relationship of this satellite DNA hypomethylation in Wilms tumors to chromosome aberrations, as determined by assays for loss of heterozygosity. (C) Elsevier Science Inc., 1999. All rights reserved.