Calculation of transmitter concentration in individual PC12 cell vesicles with electrochemical data and a distribution of vesicle size obtained by electron microscopy
Calculation of transmitter concentration in individual PC12 cell vesicles with electrochemical data and a distribution of vesicle size obtained by electron microscopy
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DOI：
10.1016/s0165-0270(99)00023-0
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发表时间：
1999-05-01
影响因子：
3
通讯作者：
Ewing, AG
中科院分区：
文献类型：
--
作者：
Anderson, BB;Zerby, SE;Ewing, AG
描述了一个数学模型，以准确地计算囊泡的大小和神经递质的浓度分布从电化学数据。该模型使用从培养中的PC 12细胞获得的电化学胞吐数据的参数来计算尺寸分布，然后将其与通过电子显微镜获得的囊泡的尺寸相关联。从电化学数据计算的尺寸分布的相对标准偏差为25%，这与通过电子显微镜测量的囊泡尺寸分布的相对标准偏差相匹配。将从电化学数据计算的分布归一化为从电子显微镜获得的PC 12细胞囊泡的囊泡尺寸分布。从归一化的大小数据和电化学参数的囊泡儿茶酚胺浓度直方图的计算，然后可能为个人胞吐事件。通过该方法计算的PC 12细胞的平均囊泡儿茶酚胺浓度为148 +/- 7 mM。更重要的是，存在浓度分布而不是恒定值。此外，该模型允许评价发射器的浓度在每个单独的囊泡和囊泡大小为每个囊泡从电化学数据时，整体囊泡大小分布是已知的。(C)1999 Elsevier Science B. V.保留所有权利。
A mathematical model is described to accurately calculate vesicle size and neurotransmitter concentration distributions from electrochemical data. This model uses parameters from electrochemical exocytosis data obtained from PC12 cells in culture to calculate a size distribution that is then correlated to the size of vesicles obtained by electron microscopy. The relative standard deviation of the size distribution calculated from electrochemical data is 25% which matches the relative standard deviation of the vesicle size distribution measured by electron microscopy. The distribution calculated from electrochemical data is normalized to the vesicle size distribution of PC12 cell vesicles obtained from electron microscopy. Calculation of a vesicular catecholamine concentration histogram from the normalized size data and electrochemical parameters is then possible for individual exocytosis events. The average vesicular catecholamine concentration for PC12 cells as calculated by this method is 148 +/- 7 mM. More importantly, there is a distribution of concentration rather than a constant value. Additionally, the model permits evaluation of the concentration of transmitter in each individual vesicle and vesicle size for each vesicle from electrochemical data when the overall vesicle size distribution is known. (C) 1999 Elsevier Science B.V. All rights reserved.