INTERACTIONS BETWEEN MACROPHAGES OF GUINEA-PIGS AND SALMONELLAE .3. BACTERICIDAL ACTION AND CYTOPHILIC ANTIBODIES OF MACROPHAGES OF INFECTED GUINEA-PIGS
INTERACTIONS BETWEEN MACROPHAGES OF GUINEA-PIGS AND SALMONELLAE .3. BACTERICIDAL ACTION AND CYTOPHILIC ANTIBODIES OF MACROPHAGES OF INFECTED GUINEA-PIGS
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DOI：
10.1128/iai.8.2.165-172.1973
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发表时间：
1973-01-01
影响因子：
3.1
通讯作者：
MAYO, DR
中科院分区：
文献类型：
--
作者：
HSU, HS;MAYO, DR
用悬浮细胞培养法研究了鼠伤寒沙门氏菌在豚鼠巨噬细胞内的去向。本研究证实正常豚鼠的巨噬细胞能够灭活摄入的沙门氏菌。先前感染的豚鼠的巨噬细胞清除摄入病原体的能力没有明显增加。然而，观察到免疫巨噬细胞在接触沙门氏菌时紧密聚集在一起。这一现象归因于免疫巨噬细胞上存在特定的嗜细胞抗体。用硫代巴比妥灭活免疫巨噬细胞时，免疫巨噬细胞与OFS的H抗原和O抗原均有凝集作用。鼠伤寒沙门氏菌与其它沙门氏菌的O抗原，而不与大肠杆菌的O抗原。在无免疫血清的情况下，免疫巨噬细胞可通过培养洗脱嗜细胞抗体。相反，在免疫血清存在的情况下，亲细胞抗体可以被动地转移到正常巨噬细胞上。此外，使用预先吸附了OFS抗原的免疫血清。抗H抗原的亲细胞抗体可单独转移到正常巨噬细胞，或仅抗O抗原的抗体可从免疫巨噬细胞中洗脱。这些数据表明，免疫巨噬细胞具有针对OFS的H和O抗原的特异性嗜细胞抗体。免疫巨噬细胞上的嗜细胞抗体的存在代表了一种通过增强巨噬细胞的聚集和吞噬活性而表现出的抗菌细胞免疫。
The fate of virulentSalmonella typhimuriumwithin macrophages of guinea pigs was assessed by a suspended cell culture procedure. The present study confirmed that macrophages of normal guinea pigs were capable of inactivating the ingested salmonellae. Macrophages of previously infected guinea pigs were not endowed with any significant increase in their ability to eliminate the ingested pathogen. However, the immune macrophages were observed to clump together tightly when they were exposed to salmonellae. This phenomenon was attributed to the presence of specific cytophilic antibodies on the immune macrophages. When immune macrophages were inactivated with Merthiolate, they agglutinated with both the H and the O antigens ofS. typhimurium, but not with the O antigens of other species ofSalmonellanor with the O antigens ofEscherichia coli.Cytophilic antibodies could be eluted from immune macrophages by incubation in the absence of immune serum. Conversely, cytophilic antibodies could be passively transferred onto normal macrophages by incubation in the presence of immune serum. Furthermore, using immune serum previously adsorbed with the O antigens ofS. typhimurium, cytophilic antibodies against the H antigens alone could be transferred onto normal macrophages, or those against the O antigens alone could be eluted from immune macrophages. These data suggest that immune macrophages possess specific cytophilic antibodies against both the H and the O antigens ofS. typhimurium.It is proposed that the presence of cytophilic antibodies on immune macrophages represents an expression of antibacterial cellular immunity by enhanced clumping and phagocytic activities of the macrophages.