High diversity and complex evolution of fungal cytochrome P450 reductase: Cytochrome P450 systems
High diversity and complex evolution of fungal cytochrome P450 reductase: Cytochrome P450 systems
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DOI：
10.1016/j.fgb.2007.10.004
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发表时间：
2008-04-01
影响因子：
3
通讯作者：
Komel, Radovan
中科院分区：
文献类型：
--
作者：
Lah, Ljerka;Krasevec, Nada;Komel, Radovan
细胞色素P450还原酶（CPR）是P450单加氧酶的氧化还原伙伴，参与真核生物的初级和次级代谢。在丝状子囊菌Cochliobolus lunatus中发现了两个新的CPR基因，氨基酸同源性为34%。在真菌基因组中搜索推定的CPR酶。系统发育分析表明，多个独立的CPR重复事件发生在真菌中，而P450-CPR融合发生在Dikarya和接合菌门的多样化之前。此外，甲硫氨酸合成酶还原酶的损失被发现在某些真菌类群中，这种酶的截短形式是保守的盘菌亚门。在真菌中，大量的细胞色素P450酶，多种CPR。P450-CPR融合蛋白与丝状菌生长相关。丝状真菌中多种CPR样氧化还原酶的进化可能是由其生长形式所必需的生化功能的复杂性驱动的，而不是酵母。(C)2007年爱思唯尔公司All rights reserved.
Cytochrome P450 reductase (CPR) is the redox partner of P450 monooxygenases, involved in primary and secondary metabolism of eukaryotes. Two novel CPR genes, sharing 34% amino acid identity, were found in the filamentous ascomycete Cochliobolus lunatus. Fungal genomes were searched for putative CPR enzymes. Phylogenetic analysis suggests that multiple independent CPR duplication events occurred in fungi, whereas P450-CPR fusion occurred before the diversification of Dikarya and Zygomycota. Additionally, losses of methionine synthase reductase were found in certain fungal taxa; a truncated form of this enzyme was conserved in Pezizomycotina. In fungi, high numbers of cytochrome P450 enzymes, multiple CPRs. and P450-CPR fusion proteins were associated with filamentous growth. Evolution of multiple CPR-like oxidoreductases in filamentous fungi might have been driven by the complexity of biochemical functions necessitated by their growth form, as opposed to yeast. (C) 2007 Elsevier Inc. All rights reserved.