EPIDERMAL GROWTH-FACTOR IN HUMAN AND BOVINE-MILK
EPIDERMAL GROWTH-FACTOR IN HUMAN AND BOVINE-MILK
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DOI：
10.1111/j.1651-2227.1992.tb12227.x
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发表时间：
1992-04-01
期刊：
影响因子：
3.8
通讯作者：
SUNAHARA, GI
中科院分区：
文献类型：
--
作者：
IACOPETTA, BJ;GRIEU, F;SUNAHARA, GI
本文用放射受体法测定了人乳和牛乳中表皮生长因子的含量。人胎盘质膜和人表皮样癌细胞均用作测定中的表皮生长因子受体来源。在放射受体测定中使用胎盘质膜给出了错误的牛乳结果，并高估了人乳中表皮生长因子的浓度。完整的细胞似乎提供了一个更准确的测量牛奶样品中表皮生长因子的浓度。使用A431细胞，我们发现与人乳（30-40 ng/ml）相比，牛乳中表皮生长因子的浓度非常低（< 2 ng/ml）。在几种以牛奶为基础的婴儿配方奶粉中没有发现表皮生长因子活性。这些结果强调了在测量复杂样品（如牛奶）中的微量物质（如多肽生长因子）时必须注意的事项。
The concentration of epidermal growth factor in human and bovine milk was measured by radioreceptor assay. Both human placental plasma membranes and a human epidermoid carcinoma cell were used as the epidermal growth factor receptor source in the assay. The use of placental plasma membrane in the radioreceptor assay gave erroneous results for bovine milk and overestimated the concentration of epidermal growth factor in human milk. Intact cells appear to provide a more accurate measure of the concentration of epidermal growth factor in milk samples. Using A431 cells, we found very low concentrations of epidermal growth factor in bovine milk (< 2 ng/ml) compared to human milk (30-40 ng/ml). No epidermal growth factor activity was found in several cows' milk-based infant formulas. These results highlight the caution which must be taken when measuring trace substances such as polypeptide growth factors in complex samples such as milk.