α-Synuclein immunoreactivity in dementia with Lewy bodies:: morphological staging and comparison with ubiquitin immunostaining
α-Synuclein immunoreactivity in dementia with Lewy bodies:: morphological staging and comparison with ubiquitin immunostaining
复制标题
DOI：
10.1007/s004010051135
复制
发表时间：
2000-04-01
影响因子：
12.7
通讯作者：
Hyman, BT
中科院分区：
文献类型：
--
作者：
Gómez-Tortosa, E;Newell, K;Hyman, BT
突触核蛋白是一种突触前蛋白，最近被发现是路易小体（LB）和路易神经突的特定成分。本研究的目的是评估痴呆伴LB （DLB）患者α -突触核蛋白免疫反应性的形态和分布，并将α -突触核蛋白与泛素免疫染色进行比较。我们用双α -突触核蛋白和泛素免疫染色检测了25例符合DLB神经病理标准的患者的黑质、旁缘区（内嗅皮层、扣带回、岛和海马）和新皮层（额叶和枕叶联合皮层）。α -突触核蛋白免疫染色比泛素免疫染色更具特异性，可以将LB与球状缠结区分开来。它也稍微更敏感，能多染色4-5%的胞浆内结构，尤其是泛素阴性的弥漫性α -突触核蛋白沉积。除LB外，α -突触核蛋白染色在黑质、海马CA2-3区和嗅内皮层显示丝状和球状神经突。在黑质中可见α -突触核蛋白染色的光谱：从弥漫的“云样”包涵体到聚集的胞浆内包涵体（可变泛素染色）到典型的LB。我们假设这些代表LB形成的不同阶段。
alpha-Synuclein is a presynaptic protein recently identified as a specific component of Lewy bodies (LB) and Lewy neurites. The aim of this study was to assess the morphology and distribution of alpha-synuclein immunoreactivity in cases of dementia with LB (DLB), and to compare alpha-synuclein with ubiquitin immunostaining. We examined substantia nigra, paralimbic regions (entorhinal cortex, cingulate gyrus, insula and hippocampus), and neocortex (frontal and occipital association cortices) with double alpha-synuclein and ubiquitin immunostaining in 25 cases meeting neuropathological criteria for DLB. alpha-Synuclein immunostaining was more specific than ubiquitin immunostaining in that it differentiated LB from globose tangles. It was also slightly more sensitive, staining 4-5% more intracytoplasmic structures, especially diffuse alpha-synuclein deposits that were ubiquitin negative. In addition to LB, alpha-synuclein staining showed filiform and globose neurites in the substantia nigra, CA2-3 regions of the hippocampus, and entorhinal cortex. A spectrum of alpha-synuclein staining was seen in substantia nigra: from diffuse "cloud-like" inclusions to aggregated intracytoplasmic inclusions with variable ubiquitin staining to classic LB. We hypothesize that these represent different stages in LB formation.