Structural specializations emerging late in mouse lens fiber cell differentiation
Structural specializations emerging late in mouse lens fiber cell differentiation
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DOI：
10.1167/iovs.07-0109
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发表时间：
2007-07-01
影响因子：
4.4
通讯作者：
FitzGerald, Paul
中科院分区：
文献类型：
--
作者：
Blankenship, Tom;Bradshaw, Linsey;FitzGerald, Paul
目的.目的：描述小鼠透镜纤维细胞中一种以前未发现的结构特化，并描述其出现与透镜发育和纤维细胞分化的关系。透镜固定效率采用C-14-甲醛和放射自显影进行了研究。透镜纤维细胞结构通过扫描电子显微镜和甲基丙烯酸酯切片的DiI标记进行检查，范围从2周到8个月。扫描电子显微镜发现了一个精心设计的结构专业化出现在纤维细胞分化后期，主要是在细胞已经失去了它的细胞核。这些精细结构从细胞的短边突出，在细胞的整个中心区域有规律地间隔开，并与相邻细胞中的类似结构对齐。在两周龄以下晶状体的纤维细胞中未发现这种结构，在此之前开始分化的纤维细胞中也未发现这种结构。2周龄后出现的纤维细胞经历了一个结构分化程序，这与发育早期出现的细胞不同。该程序包括组装一系列规则间隔的，复杂的，从纤维细胞的横向突起，使其与相邻细胞中的类似结构对齐。大多数（如果不是全部的话）结构特化发生在已经失去细胞核和细胞器的细胞中，这表明纤维细胞分化的这一组分可能不需要持续的转录/翻译。
PURPOSE. To describe a previously uncharacterized structural specialization in the mouse lens fiber cell and to delineate its emergence relative to lens development and fiber cell differentiation.METHODS. Lens fixation efficiency was explored using C-14-formaldehyde and autoradiography. Lens fiber cell architecture was examined by scanning electron microscopy and by DiI labeling of methacrylate sections in lenses ranging from 2 weeks to 8 months.RESULTS. Scanning electron microscopy identified an elaborate structural specialization that emerges late in fiber cell differentiation, largely after the cell has lost its nucleus. These elaborations project from the short side of the cell, are regularly spaced throughout the central region of the cell and are aligned with similar structures in adjacent cells. The structures are not found in fiber cells of lenses younger than two weeks of age, nor in the fiber cells that initially differentiate before that time.CONCLUSIONS. Fiber cells that arise later than 2 weeks of age undergo a structural differentiation program that is different from that of cells that arise earlier in development. This program includes the assembly of a series of regularly spaced, complex, lateral projections from the fiber cell that align themselves with similar structures in adjacent cells. Most if not all of the structural specialization occurs in cells that have lost their nuclei and organelles, suggesting that this component of fiber cell differentiation may not require ongoing transcription/translation.