Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes
Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes
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DOI：
10.1128/jcm.41.3.1311-1315.2003
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发表时间：
2003-03-01
影响因子：
9.4
通讯作者：
Cambau, E
中科院分区：
文献类型：
--
作者：
Dauendorffer, JN;Guillemin, I;Cambau, E
DNA旋切酶A亚基和B亚基喹诺酮类药物耐药区（QRDRs）氨基酸序列的测定是检测分枝杆菌氟喹诺酮类药物耐药性的分子检测方法。我们观察是否可以通过分析相应的核苷酸序列同时获得分枝杆菌种类的分配。对临床实验室常见分离的21种分枝杆菌133株参考菌株和临床菌株gyrA和gyrB QRDRs进行PCR测序。gyrA和gyrB QRDRs的核苷酸序列具有物种特异性，与氟喹诺酮类药物的敏感性无关。
The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance in mycobacteria. We looked to see if the assignment of mycobacterial species could be obtained simultaneously by analysis of the corresponding nucleotide sequences. PCR sequencing of gyrA and gyrB QRDRs was performed for 133 reference and clinical strains of 21 mycobacterial species commonly isolated in clinical laboratories. Nucleotide sequences of gyrA and gyrB QRDRs were species specific, regardless of fluoroquinolone susceptibility.