Cell-cell communication in nematode embryos: Differences between Cephalobus spec and Caenorhabditis elegans
Cell-cell communication in nematode embryos: Differences between Cephalobus spec and Caenorhabditis elegans
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DOI：
10.1007/s004270050027
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发表时间：
1996-06-01
影响因子：
2.4
通讯作者：
Schierenberg, E
中科院分区：
文献类型：
--
作者：
Bossinger, O;Schierenberg, E
在早期线虫胚胎发生过程中，生殖细胞系中的一系列不对称分裂产生了几个体细胞创始细胞和一个原始生殖细胞。我们以前已经发现，两种土壤线虫Cearibus spec.和Caaribhabditis elegans在早期胚胎发生过程中的事件顺序和空间排列的细胞表现出相当大的差异。借助显微注射荧光标记染料，我们在这里表明，这些不同的合作伙伴在细胞间通讯模式的主要差异。而在C。在线虫中，所有早期的卵裂球都同时发生染料偶联，而在哺乳动物中，通讯是按照细胞出生的顺序逐步建立的。此外，在Cephalus而不是C.在elegans中，连续的荧光黄积累表明早期卵裂球状态的逐步变化：例如，如果注射到未分裂的受精卵中，染料首先变得均匀地分布到所有细胞中，但在4细胞阶段迅速积累在单个卵裂球中。我们推测，这样的再分配机制可能参与细胞质成分的差异分离到单个卵裂球。这两个物种之间最显着的差异被发现与高分子量分子的转移。与C.在线虫中，不仅小荧光染料而且高分子量葡聚糖都可以沿着沿着特定途径在早期体细胞之间扩散，表明存在大的通讯通道。然而，葡聚糖转移到生殖系细胞中或从生殖系细胞中转移出来从未发生。这些渠道的起源，以前的有丝分裂和正常发展的潜在作用的中间体进行了讨论。组织特异性染料偶联室在缓慢发展的Ceanobus建立在相同的顺序，但在一个相当早的发展阶段比C。elegans的研究表明，这一过程可能更多地取决于转录可用时间等参数，而不是细胞周期的数量。
During early nematode embryogenesis a series of asymmetric cleavages in the germ line generates several somatic founder cells and a primordial germ cell. We have found previously that the two soil nematodes Cephalobus spec. and Caenorhabditis elegans express considerable differences in the order of events and spatial arrangement of cells during early embryogenesis. With the help of microinjected fluorescent marker dyes, we show here that these dissimilarities partner major differences in the pattern of intercellular communication. Whilst in C. elegans all early blastomeres become dye-coupled simultaneously, in Cephalobus communication is established progressively in the sequence in which cells are born. In addition, in Cephalobus but not C. elegans, sequential lucifer yellow accumulation indicates stepwise changes in the state of early blastomeres: if injected into the uncleaved zygote, for example, the dye becomes equally distributed to all cells at first but rapid ly accumulates in a single blastomere in the 4-cell stage. We speculate that such a redistribution mechanism may be involved in the differential segregation of cytoplasmic components to individual blastomeres. The most dramatic difference between the two species was found with respect to the transfer of high molecular weight molecules. In contrast to C. elegans, in Cephalobus not only small lucifer dyes but also high molecular weight dextrans can diffuse along specific pathways between early somatic cells indicating the presence of large communication channels. However, a transfer of dextran into or out of germ line cells never takes place. The origin of these channels as midbodies of previous mitoses and their potential role for normal development is discussed. Tissue-specific dye-coupling compartments in the slow developing Cephalobus are established in the same order but at a considerably earlier developmental stage than in C. elegans suggesting that this process may depend more on parameters like available time for transcription rather than the number of cell cycles passed through.