Passive Exercise Using Whole-Body Periodic Acceleration Enhances Blood Supply to Ischemic Hindlimb
Passive Exercise Using Whole-Body Periodic Acceleration Enhances Blood Supply to Ischemic Hindlimb
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DOI：
10.1161/atvbaha.111.229773
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发表时间：
2011-12-01
影响因子：
8.7
通讯作者：
Ogawa, Hisao
中科院分区：
文献类型：
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作者：
Rokutanda, Taku;Izumiya, Yasuhiro;Ogawa, Hisao
全身周期性加速度（WBPA）已被开发为一种被动运动技术，通过在脊柱轴方向上重复运动增加剪切应力来改善内皮功能。我们调查了影响WBPA对血流量恢复的小鼠模型后肢缺血和外周动脉diseases.Methods和Results-After单侧股动脉切除，小鼠被分配到无论是WBPA（n = 15）或对照组（n = 13）。在麻醉下每天一次以150 cpm施加WBPA 45分钟。通过激光多普勒灌注成像确定，WBPA显著增加缺血手术后的血流恢复。用抗CD 31抗体染色的缺血性闭肌切片显示，与对照小鼠相比，WBPA小鼠的毛细血管密度显著增加。WBPA增加骨骼肌内皮型一氧化氮合酶（eNOS）的磷酸化。在eNOS缺陷小鼠中，WBPA对缺血肢体的促血管生成作用减弱，表明WBPA对血管再生的刺激作用是eNOS依赖的。实时定量聚合酶链反应分析显示，WBPA显著增加了缺血后肢血管生成生长因子的表达。在糖尿病小鼠模型中观察到促进血流恢复，尽管葡萄糖耐量和胰岛素敏感性没有变化。此外，无论是一个单一的会议和7天的重复会议的WBPA显着改善外周动脉diseases.Conclusion-WBPA通过激活eNOS信号和上调促血管生成生长因子在缺血性骨骼肌的下肢血流量增加缺血下肢的血液供应。WBPA是一种潜在的合适的非侵入性干预，以促进治疗性血管生成。（Arterioscler Thromb Vasc Biol.2011;31：2872-2880.）
Objective-Whole-body periodic acceleration (WBPA) has been developed as a passive exercise technique to improve endothelial function by increasing shear stress through repetitive movements in spinal axis direction. We investigated the effects of WBPA on blood flow recovery in a mouse model of hindlimb ischemia and in patients with peripheral arterial disease.Methods and Results-After unilateral femoral artery excision, mice were assigned to either the WBPA (n = 15) or the control (n = 13) group. WBPA was applied at 150 cpm for 45 minutes under anesthesia once a day. WBPA significantly increased blood flow recovery after ischemic surgery, as determined by laser Doppler perfusion imaging. Sections of ischemic adductor muscle stained with anti-CD31 antibody showed a significant increase in capillary density in WBPA mice compared with control mice. WBPA increased the phosphorylation of endothelial nitric oxide synthase (eNOS) in skeletal muscle. The proangiogenic effect of WBPA on ischemic limb was blunted in eNOS-deficient mice, suggesting that the stimulatory effects of WBPA on revascularization are eNOS dependent. Quantitative real-time polymerase chain reaction analysis showed significant increases in angiogenic growth factor expression in ischemic hindlimb by WBPA. Facilitated blood flow recovery was observed in a mouse model of diabetes despite there being no changes in glucose tolerance and insulin sensitivity. Furthermore, both a single session and 7-day repeated sessions of WBPA significantly improved blood flow in the lower extremity of patients with peripheral arterial disease.Conclusion-WBPA increased blood supply to ischemic lower extremities through activation of eNOS signaling and upregulation of proangiogenic growth factor in ischemic skeletal muscle. WBPA is a potentially suitable noninvasive intervention to facilitate therapeutic angiogenesis. (Arterioscler Thromb Vasc Biol. 2011;31:2872-2880.)