Arabidopsis chloroplast quantitative editotype
Arabidopsis chloroplast quantitative editotype
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DOI：
10.1016/j.febslet.2013.03.022
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发表时间：
2013-05-02
期刊：
影响因子：
3.5
通讯作者：
Stern, David B.
中科院分区：
文献类型：
--
作者：
Ruwe, Hannes;Castandet, Benoit;Stern, David B.
叶绿体C-to-U RNA编辑是一个重要的转录后过程。在这里，我们使用来自野生型和RNA 3'末端成熟缺陷突变体的链特异性深度测序数据集分析了拟南芥中的RNA编辑。我们证明了所有位点的编辑都是部分的，平均有5-6%的RNA未被编辑。此外，我们确定了9个新的网站，编辑程度低。其中，WT转录组中不存在三个位点，因为它们被3'端RNA加工去除，但这些区域在缺乏多核苷酸磷酸化酶的突变体中积累并被编辑。(C)2013年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Chloroplast C-to-U RNA editing is an essential post-transcriptional process. Here we analyzed RNA editing in Arabidopsis thaliana using strand-specific deep sequencing datasets from the wild-type and a mutant defective in RNA 3' end maturation. We demonstrate that editing at all sites is partial, with an average of 5-6% of RNAs remaining unedited. Furthermore, we identified nine novel sites with a low extent of editing. Of these, three sites are absent from the WT transcriptome because they are removed by 3' end RNA processing, but these regions accumulate, and are edited, in a mutant lacking polynucleotide phosphorylase. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.