Metabolic engineering of Escherichia coli for L-tyrosine production by expression of genes coding for the chorismate mutase domain of the native chorismate mutase-prephenate dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas mobilis
Metabolic engineering of Escherichia coli for L-tyrosine production by expression of genes coding for the chorismate mutase domain of the native chorismate mutase-prephenate dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas mobilis
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DOI：
10.1128/aem.02456-07
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发表时间：
2008-05-01
影响因子：
4.4
通讯作者：
Gosset, Guillermo
中科院分区：
文献类型：
--
作者：
Chavez-Bejar, Maria I.;Lara, Alvaro R.;Gosset, Guillermo
将来自运动发酵单胞菌的反馈抑制不敏感酶环己二烯基脱氢酶 (TyrC) 和来自大肠杆菌的天然分支酸变位酶-预苯酸脱水酶 (PheA(CM)) 的分支酸变位酶结构域的表达与天然反馈抑制敏感的分支酸变位酶-预苯酸脱氢酶的表达进行比较 (CM-TyrA(P)) 关于在经过修饰以增加分支酸碳流的大肠杆菌菌株中产生 L-酪氨酸的能力。摇瓶实验表明，与 CM-TyrA(P) 获得的产量相比，TyrC 使从葡萄糖 (YL-Tyr/Glc) 获得的 L-酪氨酸产量增加了 6.8 倍。在生物反应器实验中，表达 TyrC 和 PheA(CM) 的菌株产生 3 g/升的 L-酪氨酸，YL-Tyr/Glc 为 66 mg/g。这些值比仅表达 TyrC 的菌株的值高 46% 和 48%。结果表明，反馈抑制不敏感酶可用于菌株开发，作为 L-酪氨酸生产代谢工程策略的一部分。
The expression of the feedback inhibition-insensitive enzyme cyclohexadienyl dehydrogenase (TyrC) from Zymomonas mobilis and the chorismate mutase domain from native chorismate mutase-prephenate dehydratase (PheA(CM)) from Escherichia coli was compared to the expression of native feedback inhibition-sensitive chorismate mutase-prephenate dehydrogenase (CM-TyrA(P)) with regard to the capacity to produce L-tyrosine in E. coli strains modified to increase the carbon flow to chorismate. Shake flask experiments showed that TyrC increased the yield of L-tyrosine from glucose (YL-Tyr/Glc) by 6.8-fold compared to the yield obtained with CM-TyrA(P). In bioreactor experiments, a strain expressing both TyrC and PheA(CM) produced 3 g/liter of L-tyrosine with a YL-Tyr/Glc of 66 mg/g. These values are 46 and 48% higher than the values for a strain expressing only TyrC. The results show that the feedback inhibition-insensitive enzymes can be employed for strain development as part of a metabolic engineering strategy for L-tyrosine production.