Regulation of cyclic longitudinal mechanical stretch on proliferation of human bone marrow mesenchymal stem cells.
Regulation of cyclic longitudinal mechanical stretch on proliferation of human bone marrow mesenchymal stem cells.
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DOI：
10.3970/mcb.2007.004.201
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发表时间：
2007-12
期刊：
影响因子：
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通讯作者：
Guanbin Song;Y. Ju;H. Soyama;T. Ohashi;Masaaki Sato
中科院分区：
文献类型：
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作者：
Guanbin Song;Y. Ju;H. Soyama;T. Ohashi;Masaaki Sato
机械刺激对于活细胞的生理和病理状态都是至关重要的。尽管对骨髓间充质干细胞（MSCs）的生物学和生化调控已经做了大量的研究，但生物力学因素对其行为的影响仍然没有完全记录。在这项研究中，我们研究了机械拉伸的幅度，频率和持续时间的调制对人骨髓间充质干细胞（hMSCs）增殖的体外模型系统使用机械拉伸加载装置，并优化拉伸制度的hMSCs的增殖。我们应用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四溴噻唑（MTT）测定来评估牵张对hMSCs的总体增殖作用。我们发现纤连蛋白涂层增加了与硅胶腔表面的粘附，但对hMSCs的增殖没有显着影响。1 Hz的频率更有效地刺激hMSCs增殖。1Hz、5%应变作用15、30、60 min，hMSCs增殖明显增加。1Hz、10%应变15、30 min时细胞增殖增强，60 min时细胞增殖减弱; 1Hz、15%应变15 min时细胞增殖减弱，30 min和60 min时细胞增殖增强。长时间（12和24 h）应用菌株抑制增殖。这些结果表明，机械牵张在hMSCs的生长和增殖中起着重要作用，适当的机械牵张机制可能是促进hMSCs体外增殖的一种新方法。
Mechanical stimulation is critical to both physiological and pathological states of living cells. Although a great deal of research has been done on biological and biochemical regulation of the behavior of bone marrow mesenchymal stem cells (MSCs), the influence of biomechanical factors on their behavior is still not fully documented. In this study, we investigated the modulation of mechanical stretch magnitude, frequency, and duration on the human marrow mesenchymal stem cells (hMSCs) proliferation by an in vitro model system using a mechanical stretch loading apparatus, and optimized the stretch regime for the proliferation of hMSCs. We applied 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrasodium bromide (MTT) assay to estimate the overall proliferative effects of the stretch on hMSCs. We found that fibronectin coating increased adhesion to silicone chamber surface, however, it did not show significant effect on proliferation of hMSCs. A frequency of 1 Hz was more effective in stimulating hMSCs proliferation. At 1 Hz, 5% strain for 15, 30, 60 min, the significant increase of hMSCs proliferation was observed. Proliferation was enhanced at 1 Hz, 10% strain for 15, 30 min, while decreased for 60 min. At 1 Hz, 15% strain, 15 min stretch resulted in the decrease of proliferation, and 30 min and 60 min stretch showed an increased proliferation. Long time (12 and 24 h) strain application blocked the proliferation. These results indicate that mechanical stretch plays an important role in hMSCs growth and proliferation; an appropriate mechanical stretch regime could be a novel approach to promoting proliferation of hMSCs in vitro.