Oriented immobilization of IgG on hydroxylated Si(001) surfaces via protein-A by a multiple-step process based on a self-assembly approach
Oriented immobilization of IgG on hydroxylated Si(001) surfaces via protein-A by a multiple-step process based on a self-assembly approach
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DOI：
10.1007/s10853-007-1809-1
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发表时间：
2007-11-01
影响因子：
4.5
通讯作者：
Piskin, E.
中科院分区：
文献类型：
--
作者：
Demirel, G.;Caglayan, M. O.;Piskin, E.
本研究的目的是在硅表面上的IgG分子的定向固定。本研究采用多步法定向固定IgG。在Si（001）表面羟基化后，3-缩水甘油氧基丙基三甲氧基硅烷（GPTS）分子自组装到这些衬底上。浸渍时间和GPTS浓度受到层厚度和水接触角的影响。然后用GPTS分子将2，2 '-（亚乙二氧基）二乙胺（EDA）分子共价连接到硅表面。浓度对EDA分子在表面上的形成没有影响，但EDA沉积随着浸渍时间的增加而显著增加。成像椭圆偏振法和原子力显微镜（AFM）图像显示在这一步聚集体的形成。通过使用碳二亚胺（即，EDAC）作为活化剂。我们能够将IgG分子以定向形式固定在蛋白A附着的表面上，特别是在EDA聚集体所在的区域。
The aim of this study was the oriented immobilization of IgG molecules on the silicon surfaces. A multiple-step procedure was applied for oriented immobilization of IgG in this study. After hydroxylation of the Si(001) surfaces, 3-glycidoxypropyltrimethoxysilane (GPTS) molecules were self-assembled onto these substrates. Dipping time and GPTS concentration were found to be effected by on both layer thicknesses and water-contact angles. 2,2'-(ethylenedioxy)diethylamine (EDA) molecules were then covalently attached to the silicon surface with GPTS molecules. There was no effect of concentration on the formation of EDA molecules on the surfaces, while EDA deposition increased with the dipping time significantly. Imaging ellipsometry and atomic force microscopy (AFM) images exhibited aggregate formation at this step. Protein-A molecules were bound to the free amino groups of EDA molecules on the substrate surface, especially onto the aggregates by using a carbodiimide (i.e., EDAC) as the activating agent. We were able to immobilize IgG molecules in an oriented form onto the protein-A attached surfaces, especially in the regions, where EDA aggregates are located.