SIMPLE, EFFICIENT INVITRO SYNTHESIS OF CAPPED RNA USEFUL FOR DIRECT EXPRESSION OF CLONED EUKARYOTIC GENES
SIMPLE, EFFICIENT INVITRO SYNTHESIS OF CAPPED RNA USEFUL FOR DIRECT EXPRESSION OF CLONED EUKARYOTIC GENES
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DOI：
10.1093/nar/10.20.6353
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发表时间：
1982-01-01
影响因子：
14.9
通讯作者：
FIERS, W
中科院分区：
文献类型：
--
作者：
CONTRERAS, R;CHEROUTRE, H;FIERS, W
A simple and efficient method for directin.vitrosynthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts were translatedinvivoinXenopuslaevisoocytes andinvitroin reticulocyte and in wfieat germ cell-free protein synthesizing systems.
