Differential effects of dietary BHA on hepatic enzyme activities and benzo[a]pyrene metabolism in male and female NMRI mice.
Differential effects of dietary BHA on hepatic enzyme activities and benzo[a]pyrene metabolism in male and female NMRI mice.
复制标题
膳食 BHA 对雄性和雌性 NMRI 小鼠肝酶活性和苯并[a]芘代谢的不同影响。
DOI：
10.1093/carcin/3.1.15
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发表时间：
1982
期刊：
影响因子：
4.7
通讯作者：
Moldéus,P
中科院分区：
文献类型：
--
作者：
Dock,L;Cha,YN;Jernström,B;Moldéus,P
比较了2(3)-叔丁基-4-羟基苯甲醚（BHA）单独和联合腹腔注射3-甲基胆蒽（MC）对雄性和雌性NMRI小鼠肝脏酶活性和苯并[a]芘（BP）代谢的影响。总的来说，雌性小鼠在饮食中给予BHA后的特征性诱导模式在雄性小鼠中也可以看到。然而，饲喂BHA后，雄性小鼠环氧化物水解酶和胞质谷胱甘肽s -转移酶的增加并不像雌性小鼠那么明显。此外，MC处理似乎抵消了BHA在雌性中诱导的GST活性，但在雄性中没有这种效果。未经处理的雄性小鼠肝微粒体催化BP代谢的效率低于雌性小鼠。BHA处理可在相当程度上增加两性的这种活性，并且接受MC治疗的男性和女性的总体活性相同。BHA处理后，BP代谢物的模式发生了改变。总的来说，观察到bp -4,5-二醇升高，9-OH-BP降低。当比较MC处理和MC + BHA处理动物的微粒体时，也注意到这种模式。单独的MC处理增加了bp -7,8-二醇、醌类和酚类的含量。本报告表明，几个因素可能有助于小鼠对饮食中的BHA的反应。这是否对该化合物的抗癌作用有任何影响仍有待阐明。
The effect of dietary 2(3)-tert-butyl-4-hydroxy-anisole (BHA) alone and in combination with intraperitoneal injections of 3-methylcholanthrene (MC), on hepatic enzyme activities and benzo[a]pyrene (BP) metabolism was compared in male and female NMRI mice. In general, the characteristic induction pattern following dietary BHA administration in female mice could also be seen when male mice were used. The increase in epoxide hydrolase and cytosolic glutathione S-transferase following BHA feeding was however not as pronounced in males as in females. Also, MC treatment appeared to counteract the induction of GST activity by BHA in females but had no such effect in males. Liver microsomes from untreated male mice catalyzed the metabolism of BP less efficiently than did microsomes from females. BHA treatment increased this activity in both sexes to a comparable extent and the overall activity was the same in males and females having received MC. The pattern of BP metabolites was altered following BHA treatment. In general, an increase in BP-4,5-diol and a decrease in 9-OH-BP was observed. This pattern was also noticed when microsomes from MC treated and MC + BHA treated animals were compared. MC treatment alone increased the amount of BP-7,8-diol, the quinones and the phenols. The present report indicates that several factors may contribute to the response to dietary BHA in mice. Whether this has any consequence in regard to this compound's anticarcinogenic effect remains to be elucidated.