Degradation of a collagen-chondroltin-6-sulfate matrix by collagenase and by chondroitinase
Degradation of a collagen-chondroltin-6-sulfate matrix by collagenase and by chondroitinase
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DOI：
10.1016/s0142-9612(03)00541-6
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发表时间：
2004-02-01
期刊：
影响因子：
14
通讯作者：
Gibson, LJ
中科院分区：
文献类型：
--
作者：
Pek, YS;Spector, M;Gibson, LJ
通过冷冻干燥技术生产的高度多孔的I型胶原-软骨素-6-硫酸盐（胶原-GAG）支架已被证明作为促进某些组织再生的植入物具有价值。本项目的目的是评价所选胶原蛋白-GAG支架在体外模型系统中降解时的微观结构和机械性能的变化。环境扫描电子显微镜和视频成像表明，胶原酶降解通过在2小时内产生1-3 μ m直径的微坑引起支柱侵蚀，导致最终移除支柱材料和支柱断裂。显微结构形貌的损失可能是由于胶原蛋白被胶原酶切割时的凝胶化。GAG的软骨素酶降解导致支柱肿胀，导致孔变得更小和更圆。胶原蛋白-GAG基质的压缩模量下降时，降解胶原酶，但保持不变时，降解chondroitinase. Carbodimide-crosslinked矩阵被发现有一个更高的交联密度，更高的压缩刚度和更大的阻力胶原酶和chondroitinase，相比，非交联对照和矩阵的交联的脱水过程。本研究提供了可用于设计具有所需压缩刚度和对胶原酶和软骨素酶的降解速率的胶原-GAG支架的信息。(C)2003爱思唯尔有限公司。保留所有权利。
Highly porous, type I collagen-chondroitin-6-sulfate (collagen-GAG) scaffolds, produced by freeze-drying techniques, have proven to be of value as implants to facilitate the regeneration of certain tissues. The objective of this project was to evaluate changes in the microstructure and mechanical properties of selected collagen-GAG scaffolds as they degrade in an in vitro model system. Environmental scanning electron microscopy and video imaging demonstrated that collagenase degradation caused strut erosion through the creation of 1-3 mum diameter micropits within a 2-h period, leading to eventual removal of strut material and strut breakage. Loss of microstructural topography may have been due to gelatinization when collagen was cleaved by collagenase. Chondroitinase degradation of GAG resulted in swelling of the struts, causing the pores to become smaller and rounder. The compressive modulus of the collagen-GAG matrix decreased when degraded by collagenase, but remained unchanged when degraded by chondroitinase.Carbodiimide-cross-linked matrices were found to have a higher cross-link density, a higher compressive stiffness and a greater resistance to collagenase and chondroitinase, compared to non-cross-linked controls and matrices that were cross-linked by the dehydrothermal process. This investigation provides information that can be used to design collagen-GAG scaffolds with desired compressive stiffness and degradation rate to collagenase and chondroitinase. (C) 2003 Elsevier Ltd. All rights reserved.