Myofibrillar architecture in engineered cardiac myocytes.
Myofibrillar architecture in engineered cardiac myocytes.
复制标题
工程心肌细胞中的肌原纤维结构。
DOI：
10.1161/circresaha.108.182469
复制
发表时间：
2008-08-15
影响因子：
20.1
通讯作者：
Tung L
中科院分区：
文献类型：
--
作者：
Parker KK;Tan J;Chen CS;Tung L
形态发生通常被认为是转录同步和扩散有丝分裂原的空间限制的功能，然而，细胞微环境的物理约束代表了调节亚细胞结构自组装的额外机制。我们问，如果肌细胞的形状是一个独特的信号，加强组织的肌原纤维阵列在心肌肌细胞。我们设计了新生大鼠心室肌细胞的形状，通过将它们培养在微制造的纤连蛋白岛上，在那里它们扩散并呈现岛的形状。肌原纤维发生后，在空间和时间上，组装独特的肌动蛋白网络的架构是可预测的形状的岛屿。随后，肌细胞的Z线在肌细胞的不同区域以不同的模式对齐和注册，从而可以明显地设计正交收缩轴。这些数据表明，肌细胞的细胞外基质的物理约束可能是肌原纤维组织的一个重要调节器。
Morphogenesis is often considered a function of transcriptional synchrony and the spatial limits of diffusing mitogens, however, physical constrainment by the cell microenvironment represents an additional mechanism for regulating self assembly of subcellular structures. We asked if myocyte shape is a distinct signal that potentiates the organization of myofibrillar arrays in cardiac muscle myocytes. We engineered the shape of neonatal rat ventricular myocytes by culturing them on microfabricated fibronectin islands, where they spread and assumed the shape of the island. Myofibrillogenesis followed, both spatially and temporally, the assembly of unique actin networks whose architecture was predictable given the shape of the island. Subsequently, the Z-lines of myocytes aligned and registered in distinct patterns in different regions of the myocytes in such a way that orthogonal axes of contraction could be distinctly engineered. These data suggests that physical constrainment of muscle cells by extracellular matrix may be an important regulator of myofibrillar organization.